Vol 2, No 2 (2007)


Specialists’ opinions. (No. 2, 2007)

Genes & Cells. 2007;2(2):6-7
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Cell transplant

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Postnatal ovogenesis and stem cells - discussion sequel

Melikhova V.S.
Genes & Cells. 2007;2(2):20-21
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Possible pathways of regener ative strategy in correction of type I diabetes mellitus by methods of cell transplantation

Zakiryanov A.R., Onishchenko N.A.


This review describes up to date concepts of pancreatic b-cell and islets of Langerhans physiological and reparative regeneration during type I diabetes mellitus. Modern approaches to recovery of lacking b-cell population with transplantation of allogenic islet cells, in vitro differentiated adult pancreatic progenitors or embryonic stem cells are considered. Possible pathways of in vivo tissue regeneration induction or immune correction in type I diabetes mellitus with bone marrow and cord blood stem cell therapy are analyzed.

Genes & Cells. 2007;2(2):23-33
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Original Study Articles

Double negative epigenetics regulation of genes in differentiation of human endothelial stem cells into endothelium

Volchkov P.Y., Lagarkova M.A., Рrokhorovich M.A., Kiselyev S.L.


Тhere are a number of genes which expression is characteristic for endothelium. Тranscription factors GAТA-2 and -3, endothelial NO synthitase (eNOS) are among them. During early embryogenesis epigenetics mechanisms of gene regulation plays an important role. Human embryonic stem cells (hESCs) represent a valuable model to study early embryogenesis events. Using the previously developed model of hESCs differentiation to functional endothelium we compared status of GAТA-2, GAТA-3 and eNOS gene's promoters methylation in hESCs, hESCs-derived purified endothelial cells and human umbilical vein endothelial cells (HUVEC). Hypermethylation of promoter regions of genes studied in hESCs correlates with gene silencing whereas hypomethylation in hESCs-derived endothelial cells and HUVECs correlated with the high level of expression. Overall our data indicate the importance of epigenetic regulation for tissue-specific differentiation and provide the evidence that in the course of in vitro differentiation ESCs undergo the same epigenetic program as differentiating cells of embryo in vivo.

Genes & Cells. 2007;2(2):34-39
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Investigation of expression and analysis of function of Sip1 gene in cerebral cortex development

Polyakov A.S., Korochkin L.I., Pavlova G.V.


Expression of SMAD interacting protein 1 (Sip1) during mouse CNS development was studied by in situ hybridization and immunohistochemistry. Starting at E12.5, Sip1 transcripts are present in a regionalized fashion and persist throughout development. Sip1 is expressed in the cortical plate, ventricular zone of the basal ganglia, thalamus, pons and midbrain, specific nuclei of the brain stem and in the dorsal part of the spinal cord. In the developing cerebral cortex, Sip1 expression shows regional specificity. In the brain of the adult mice, SIP1 expression is detected mostly in the hippocampus, dentate gyrus and white matter of the neocortex.

We investigated the expression of the Sip1 gene in the brains of newborn reeler mutants. At this stage in the wild type piriform cortex Sip1 expression is found in the most outer part. In the piriform cortex of reeler mutants Sip1 expressing cells were not located in the superficial part but diffused.

Using a Cre/loxP-based approach, we studied the effect of conditional inactivation of Sip1 gene. The results provide new evidence for the important role of Sip1 in hippocampal neurogenesis.

Genes & Cells. 2007;2(2):40-44
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Increase of adaptive reserves of the damaged myocardium to load tests after intramyocardial transplantation of various phenotypes of bone marrow stromal cells

Potapov I.V., Kirillov I.A., Onischenko N.A.


In many works it was shown that Ьone marrow stromal cells (BMSC) transplantation improves heart function. However remains unclear whether the predifferentiation could influence on the damaged myocardial function repare, especially in the increased load settings on the left ventricle (LV). In our study one of three types of autologous BMSC has been transplanted in 1 week after cryodestruction of rat myocardium in peri-infarct areas of LV: undifferentiated, cardiomyocyte-like and endothelial-like predifferentiated BMSC.In 3 weeks after transplantation LV function has been studied using method of dosed pre- and afterloads on the isolated hearts (as provided Ьy Neely preparation). It has been shown, that after all 3 types BMSC transplantation heart showed the best stability to load tests in comparison with control group. However we consider that increase of adaptive reserves of LV myocardium after transplantation all different types of BMSC was realized by various pathways in each group.

Genes & Cells. 2007;2(2):45-49
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Different bone marrow cells autotransplantation influence on morphofunctional state of myocardium after experimental infarction

Davydenko V.V., Matyukov A.A., Tsupkina N.V., Vlasov T.D., Gritsenko V.V., Kuznetsov A.A., Аmineva K.K., Deev R.V., Yalfimov A.N., Pinaev G.P.


The contribution aims to compare influence of intramyocardial autotransplantation of bone marrow multipotent mesenchymal stromal cells and bone marrow nucleated cells on a myocardium morphofunctional state after infarction. Chinchilla rabbits were exposed in the experiment. Myocardial infarction was modeled by ligating the anterior descending branch of the left coronary artery. The condition was evaluated with functional (electrocardiography, echocardiography) and morphologic methods. There were three groups of animals. To the first group animals (control, n = 13) α-МЕМ growth medium was injected into the damaged area; a culture of bone marrow multipotent mesenchymal stromal cells (2х106) was introduced to the 2nd group animals (n = 14), while the animals of the 3rd group (n = 14) being given bone marrow nucleated cells (2х106). It has been shown that intramyocardial autotransplantation of bone marrow multipotent mesenchymal stromal cells in experimental myocardial infarction resulted in ischemic area restriction, normalization of systolic function indices as well as stimulation of angiogenesis. Аt the same time intramyocardial autotransplantation of bone marrow nucleated cells was followed by an expansion of ischemic area and reduction of systolic function indices as compared with the controls, although angiogenesis activation occurred.

Genes & Cells. 2007;2(2):52-61
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Use of bone marrow stromal cells on Biosytall granules to plasty of skull bones

Deev R.V., Tsupkina N.V., Nicolaenko N.S., Pinaev G.P.


Оne of cellular technology approaches to skull bones plasty has been demonstrated in an experimental investigation. These bones are hardly able to recover, therefore with their extensive damage there is a necessity to use various plastic material.

ln the experiment Chinchilla rabbits were exposed. Вone marrow for stromal cells isolation was derived by a standard method. Having received the required expansion of cells in culture, they were allocated on granules of bone-plastic glass-ceramic material Biosit with the

diameter of 0.3-1.0 mm. This construction was inserted into a parietal bone modeled defect with its diameter of 1.0 cm. The results were assessed with light and electron scanning microscopy at 60 and 120 days. The cell culture has been stated to adhere granule surface and results in rapid bone defect recovery within the damaged area in combination with bone-plastic material.

Genes & Cells. 2007;2(2):62-67
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Cellular Matrix Made of Resorbed Polyhydroxialkanoats

Shishatskaya E.I.


The article presents the results of a study of resorbed polyhydroxialkanoats (linear polyesters of microbiologic origin) aptitude for different cellular matrix development. ln the experiment samples of high-purity polyhydroxialkanoats produced by Ralstonia eutropha B 5786 bacteria were used. A technology to produce polyhydroxialkanoats of various chemical structures is developed in the Institute of Biophysics of the Siberian branch of the Russian Academy of Sciences. Using polyhydroxialkanoats in different phase states (solutions, emulsions, powder) a structure and properties of two- and tree-dimentional matrix such as flexible transparent slick, membrane, fine fibres, microparticles, sponge, volumetric solid and porous structures are produced and studied. The matrix can be sterilized with standard methods without changing its structure, stability loss or adhesion quality deterioration as well as its fitness for growing cells in vitro. Matrix biocompatibility was assessed in cultures of fibroblasts, hepatocytes, endothelial cells and osteoblasts in vitro. Microscopy, inter vivos/alive staining with tripan blue, detection of protein and ONA synthesis by cells being cultured, as well as MMT have not shown polyhydroxialkanoat matrix to be cytotoxic in a direct contact with any type of cells used. The data obtained allow to recommend polyhydroxialkanoats - new type of thermpstable, biocompatible and resorbed polyester - for development of different constructions for cellular therapy and tissue engineering.

Genes & Cells. 2007;2(2):68-75
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IL8 matrix-metalloproteinasa concentration in blood serum in therapy with G-CSF

Raikina E.V., Rumyantsev S.A.
Genes & Cells. 2007;2(2):76-80
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Receptor expression to G-CSF and IL8 in blood cells while treating with G-CSF

Raikina E.V., Rumyantsev S.A.
Genes & Cells. 2007;2(2):81-83
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The Legal and Ethical Status of the Human Embryo

Merkel R.
Genes & Cells. 2007;2(2):84-85
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Cryoconservation of hemapoietic cord stem cells

Grishina V.V.
Genes & Cells. 2007;2(2):91-91
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Legal regulation of stem cells banks in the US and RF

Prikhodko A.V.
Genes & Cells. 2007;2(2):92-92
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Cord blood - an alternative source of hemapoeitic stem cells for transplantation

Skorobogatova E.V., Rumyantsev A.G., Maschan A.A., Dyshlevaya Z.M., Skvortsova Y.V., Balashov D.N., Trakhtman P.E., Shipitsyna I.P., Pashko Y.V., Kournikova E.E., Blagonravova O.L.
Genes & Cells. 2007;2(2):92-92
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Cord stem cells in practical medicine

Smolyaninov A.B.
Genes & Cells. 2007;2(2):93-93
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Usage of human stem cells in experimental ischemic disorders

Surkov K.G., Belova L.A., Krasnyakov V.K., Karpova N.S.
Genes & Cells. 2007;2(2):93-93
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Public bank foundation of placenta blood in the Samara region

Tumina O.V.
Genes & Cells. 2007;2(2):94-94
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Some experience in Cord blood bank foundation in RF

Feydorov V.A.
Genes & Cells. 2007;2(2):95-95
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Thesis defense (№2, 2007)

Potapov I.V.
Genes & Cells. 2007;2(2):102-102
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