Investigation of expression and analysis of function of Sip1 gene in cerebral cortex development
- Authors: Polyakov A.S.1, Korochkin L.I.1, Pavlova G.V.1
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Affiliations:
- Institute of Gene Biology, RAS
- Issue: Vol 2, No 2 (2007)
- Pages: 40-44
- Section: Original Study Articles
- URL: https://genescells.ru/2313-1829/article/view/217699
- ID: 217699
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Abstract
Expression of SMAD interacting protein 1 (Sip1) during mouse CNS development was studied by in situ hybridization and immunohistochemistry. Starting at E12.5, Sip1 transcripts are present in a regionalized fashion and persist throughout development. Sip1 is expressed in the cortical plate, ventricular zone of the basal ganglia, thalamus, pons and midbrain, specific nuclei of the brain stem and in the dorsal part of the spinal cord. In the developing cerebral cortex, Sip1 expression shows regional specificity. In the brain of the adult mice, SIP1 expression is detected mostly in the hippocampus, dentate gyrus and white matter of the neocortex.
We investigated the expression of the Sip1 gene in the brains of newborn reeler mutants. At this stage in the wild type piriform cortex Sip1 expression is found in the most outer part. In the piriform cortex of reeler mutants Sip1 expressing cells were not located in the superficial part but diffused.
Using a Cre/loxP-based approach, we studied the effect of conditional inactivation of Sip1 gene. The results provide new evidence for the important role of Sip1 in hippocampal neurogenesis.
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About the authors
A. S. Polyakov
Institute of Gene Biology, RAS
Author for correspondence.
Email: redaktor@celltranspl.ru
Laboratory of neurogenetics and genetics of development
Russian Federation, MoscowL. I. Korochkin
Institute of Gene Biology, RAS
Email: redaktor@celltranspl.ru
Laboratory of neurogenetics and genetics of development
Russian Federation, MoscowG. V. Pavlova
Institute of Gene Biology, RAS
Email: redaktor@celltranspl.ru
Laboratory of neurogenetics and genetics of development
Russian Federation, MoscowReferences
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