Increase of adaptive reserves of the damaged myocardium to load tests after intramyocardial transplantation of various phenotypes of bone marrow stromal cells
- Authors: Potapov I.V.1, Kirillov I.A.1, Onischenko N.A.1
-
Affiliations:
- Institute of transplantology and artificial organs
- Issue: Vol 2, No 2 (2007)
- Pages: 45-49
- Section: Original Study Articles
- URL: https://genescells.ru/2313-1829/article/view/217706
- ID: 217706
Cite item
Open Access
Access granted
Abstract
In many works it was shown that Ьone marrow stromal cells (BMSC) transplantation improves heart function. However remains unclear whether the predifferentiation could influence on the damaged myocardial function repare, especially in the increased load settings on the left ventricle (LV). In our study one of three types of autologous BMSC has been transplanted in 1 week after cryodestruction of rat myocardium in peri-infarct areas of LV: undifferentiated, cardiomyocyte-like and endothelial-like predifferentiated BMSC.In 3 weeks after transplantation LV function has been studied using method of dosed pre- and afterloads on the isolated hearts (as provided Ьy Neely preparation). It has been shown, that after all 3 types BMSC transplantation heart showed the best stability to load tests in comparison with control group. However we consider that increase of adaptive reserves of LV myocardium after transplantation all different types of BMSC was realized by various pathways in each group.
Full Text
About the authors
I. V. Potapov
Institute of transplantology and artificial organs
Author for correspondence.
Email: redaktor@celltranspl.ru
Laboratory of stem cell biotechnology
Russian Federation, MoscowI. A. Kirillov
Institute of transplantology and artificial organs
Email: redaktor@celltranspl.ru
Laboratory of stem cell biotechnology
Russian Federation, MoscowN. A. Onischenko
Institute of transplantology and artificial organs
Email: redaktor@celltranspl.ru
Laboratory of stem cell biotechnology
Russian Federation, MoscowReferences
- Oswald J., Boxberger S., Jorgensen В. Et al. Mesenchymal Stem Cells Can Be Differentiated Into Endothelial Cells In Vitro. Stem Cells 2004; 22: 377-84.
- Makino S., Fukuda K., Miyoshi S. et al. Cardiomyocytes can be generated from marrow stromal cells in vitro. J. Clin. Invest. 1999; 103: 697-705.
- Neely J.R., Liebermeister H., Battersby E.J., Morgan H.E. Effect of pressure development on oxygen consumption by isolated rat heart. Am. J. Physiol. 1967; 312(4): 804-14.
- Saito T., Kuang J.Q., Lin C.C., Chiu R.C. Transcoronary implantation of bone marrow stromal cells ameliorates cardiac function after myocardial infarction. J. Thorac. Cardiovasc. Surg. 2003; 126:114-23.
- Tomita S., Li R.K., Weisel R.D. etal. Atologous transplantation of bone marrow cells improves damaged heart function. Circ. 1999; 100 Suppl II: II247-II256.
- Матюков А.А., Цупкина H.B., Власов B.B. и др. Сравнение влияния интрамиокардиальной аутотрансплантации различных клеток костного мозга на репарацию миокарда кроликов после инфаркта. Клеточная трансплантология и тканевая инженерия 2007; 2(1): 48-52.
- Liu J., Hu Q., Wang Z. et al. Autologous stem cell transplantation for myocardial repair. Am. J. Physiol. Heart. Circ. Physiol. 2004; 287: H501-H511.
- Amado L.C., Saliaris A.P., Schuleri K.H. et al. Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction. PNAS 2005; 102(32): 11474-9.
- Makkar R.R., Price M.J., Lill M. et al. Intramyocardial Injection of Allogenic Bone Marrow-Derived Mesenchymal Stem Cells Without Immunosuppression Preserves Cardiac Function in a Porcine Model of Myocardial Infarction. J. Cardiovasc. Pharmacol. Ther. 2005; 10(4): 225-33.
- Silva G.V., Litovsky S., Assad J.A.R. et al. Mesenchymal Stem Cells Differentiate into an Endothelial Phenotype, Enhance Vascular Density, and Improve Heart Function in a Canine Chronic Ischemia Model. Circ. 2005; 111:150-6.
- Потапов И.В., Башкина Л.В., Онищенко H.A. и др. Характеристика насосной функции сердца после трансплантации фетальных кардиомиоцитов и мезехимальных стволовых клеток костного мозга в криоповрежденный миокард. Вестник трансплантологии и искусственных органов 2003; 3: 50-5.
- Шумаков В.И., Онищенко Н.А., Расулов М.Ф. и др. Использование предифференцированных мезенхимальных стволовых клеток костного мозга длялечения глубокихожоговыхран. Вестникхирургии им И.И. Грекова 2003; 162(4): 38-41.
- Шумаков В.И., Онищенко Н.А., Крашенинников М.Е. и др. Дифференцировка стромальных стволовых клеток костного мозга в кардиомиоцито-подобные клетки у различных видов млекопитающих. Бюл. эксперимент. биол. и мед. 2003; 4: 461-5.
- Потапов И.В., Кириллов И.А., Крашенинников М.Е. и др. Мультипотентные мезенхимальные стромальные клетки костного мозга как источник получения клеток для восстановительного морфогенеза миокарда. Тезисы Ежегодной Всероссийской и международной научной конференции «Стволовые клетки и перспектива их использования в здравоохранении»; 2006 24-26 мая; Москва.
Supplementary files
