Double negative epigenetics regulation of genes in differentiation of human endothelial stem cells into endothelium

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Тhere are a number of genes which expression is characteristic for endothelium. Тranscription factors GAТA-2 and -3, endothelial NO synthitase (eNOS) are among them. During early embryogenesis epigenetics mechanisms of gene regulation plays an important role. Human embryonic stem cells (hESCs) represent a valuable model to study early embryogenesis events. Using the previously developed model of hESCs differentiation to functional endothelium we compared status of GAТA-2, GAТA-3 and eNOS gene's promoters methylation in hESCs, hESCs-derived purified endothelial cells and human umbilical vein endothelial cells (HUVEC). Hypermethylation of promoter regions of genes studied in hESCs correlates with gene silencing whereas hypomethylation in hESCs-derived endothelial cells and HUVECs correlated with the high level of expression. Overall our data indicate the importance of epigenetic regulation for tissue-specific differentiation and provide the evidence that in the course of in vitro differentiation ESCs undergo the same epigenetic program as differentiating cells of embryo in vivo.

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P. Yu. Volchkov

Vavilov Institute of General Genetics, RAS; Institute of Gene Biology, RAS

Author for correspondence.
Russian Federation, Moscow; Moscow

M. A. Lagarkova

Vavilov Institute of General Genetics, RAS

Russian Federation, Moscow

M. A. Рrokhorovich

Institute of Genetics and Cytology, Siberian branch of RAS

Russian Federation, Novosibirsk

S. L. Kiselyev

Vavilov Institute of General Genetics, RAS

Russian Federation, Moscow


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