Vol 2, No 1 (2007)


Specialists’ opinions. (No. 1, 2007)

Genes & Cells. 2007;2(1):6-7
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Cell technology

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Bone marrow cells can be a reservoir of various neoplasm

Sergeev V.S.
Genes & Cells. 2007;2(1):11-13
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Histocompatible embrional stem cells development by parthenogenesis

Melikhova V.S.
Genes & Cells. 2007;2(1):18-19
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Isolation of pluripotent stem cells from human amniotic fluid

Melikhova V.S.
Genes & Cells. 2007;2(1):20-20
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Resident Cardiomyocyte Precursors and Myocardium Regeneration

Rubina K.A., Melikhova V.S., Parfenova E.V.


The review represents development of different views on the potential of cardial muscle tissue regeneration as well as up-to-date data on existence and functioning of resident precursors of cardiomyocytes.

Genes & Cells. 2007;2(1):29-35
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Neuroregenerative therapy of spinal cord trauma: role and perspectives of stem cells transplantation

Zaytsev A.Y., Bryukhovetsky A.S.


It is a review of the problem, modern approaches and experimental and clinical data on different stem cells transplantation in the treatment of spinal cord traumas in experimental animals and in clinical practice. Cellular technologies might be used in neurological deficiency state in spinal cord injuries, but their usage is controvresial. Possible complications of cellular therapy in experiment and clinical practice and further usage are discussed.

Genes & Cells. 2007;2(1):36-44
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Original Study Articles

Comparison of effects of bone marrow different cells intramyocardial autotransplantation on reparation of rabbit myocardium after infarction

Matyukov A.A., Tsupkina N.V., Vlasov T.D., Gritsenko V.V., Davydenko V.V., Yalfimov A.N., Pinaev G.P.


The article represents the results of comparative estimation of effects of intramyocardial autotransplantation of bone marrow pluripotent mesenchymal stromal cells and nucleated cell on reparation of myocardium after infarction. Rabbits of the Chinchilla species were used in the experiments. Myocardial infarction was modeled by ligating the anterior descending branch of the left coronary artery. The results were assessed by a scar tissue area and the index of left ventricle dilatation in 12 months after cells transplantation. To evaluate an extend of myocardial infarction the cells were stained with 1, 3, 5, tripheniltetrasole chloride. There were three groups of animals. Into the affected* zone of the 1st group animals (control, n=13) α-МЕМ medium was injected; to the animals of the 2nd group (n=14) the culture of pluripotent mesenchymal stromal cells of bone marrow was introduced (2x106), the 3-rd group animals (n=14) were given bone marrow nucleated cells (2x106). It has been shown that transplantation of pluripotent mesenchymal stromal cells of bone marrow reduces a scar area and left ventricle dilatation as compared with the control. Bone marrow nucleated cells having been transplanted, the necrosis extends, scar area increases, every cardiac chamber substantially dilates.

Genes & Cells. 2007;2(1):48-53
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Secreted transcription factor HOXB4 promotes human ESCs endothelial lineage

Lagarkova M.A., Mufasalov I.A., Voltchkov P.Y., Kiselyev S.L.


The effect of the transcription factor HOXB4 on differentiation abilities of human ESCs to endothelial lineage was the aim of the present study. HOXB4 is a multifunctional transcription factor inducing or inhibiting apoptosis, promoting or repressing cell proliferation and differentiation. HOXB4 plays an important role in development and hematopoesis. OP9 stromal cells or OP9hoxb4 cells secreting transcription factor HOXB4-TAT were co-cultured with human embryonic stem cells to induce endothelial differentiation. OP9hoxb4 feeder cells significantly promoted timing of endothelium maturation both on the level of immunological makers expression and functionally in comparison with OP9 feeder. Thus our data indicate that secreted HOXB4-TAT largely influence controlled differentiation of human ESCs to endothelial cells.

Genes & Cells. 2007;2(1):56-59
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Hypoderm reaction to material used in stomatology for tissue engineering

Guseva A.S., Ryseva L.M., Guk A.S., Rumakin V.P.


Morphologic investigations of hypoderm tissues reaction to implantation of different material which might be potentially used as scaffolds were held. 78 lab rats were used in the experiment. Several materials were tested in the experiment, such as gold, stainless steel, stainless steel covered with nitrid titanium, different plastics — ethacril, phtorax, and colorless plastic. According to the experimental results gold and phtorax are less reactogenic.

Genes & Cells. 2007;2(1):60-64
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Controlled immunocorrection and subsequent activation of bone marrow mononuclear cells improve myocardial function in chronic ischemic cardiac failure

Temnov A.A., Gureev S.V., Onishchenko N.A., Shumakov V.I.


Chronic cardiac failure is accompanied with immune dysregulation which severity predetermines the absence of clinic efficiency of autologous bone marrow stem cells (BMSCs). The effect of two-staged activation of bone marrow stem cells (at first, in vivo, then ex vivo) on left ventricle function promotion in patients with chronic cardiac failure has been studied. Two groups of patients with chronic cardiac failure took part in the investigation. The 1st group patients (control, n=50) underwent cardiopulmonary bypass. To the patients of the 2nd group (n=57) 200 mln autologous mononuclear marrow stem cells were introduced intramyocardially during the cardiopulmonary bypass. In the 2nd group patients the original immune dysregulation severity was assessed by assaying the index of white blood cells stimulation (SI) with a chemoluminescent test. In 16 patients with SI>1 and a moderate alteration of immunoassay favorable effect of BMSCs was predicted; bone marrow stem cells were predicted to produce unfavorable clinic effect in 41 patients with SI <1 and profound alterations of immunoassay. 22 patients with SI <1 had undergone immunocorrecting therapy (stem cells in vivo activation). Mononuclear stem cells of patients (n=38 with SI>1 and n=19 with SI<1) were cultured for 5-7 days (stem cells ex vivo activation). The research demostrated that BMSCs culturing in patients with favorable prognosis led to the changes of immunophenotype of bone marrow such as CD3, CD4, CD8, CD 25 increase. The control examination of the patients 6 months after autologous bone marrow cells introduction revealed that positive dynamics of the left ventricle function and the physical activity index (DASI) occurred in patients with SI>1. Controlled immunocorrection performed before derivation of BMSCs, in combination with their subsequent ex vivo activation promoted reliable improvement of functional characteristics of the left ventricle 6 months after cardiopulmonary bypass in comparison with the controls. For autologous bone marrow stem cells to be effective in patients with chronic cardiac failure two-staged activation of BMSCs should be performed: at first, in vivo with immunocorrectors, then ex vivo while culturing.

Genes & Cells. 2007;2(1):65-73
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Clinical experience

Therapeutic effects of systemic transplantation of cultured autologous mesenchymal stem cells derived from bone marrow in patients with resistant pulmonary tuberculosis

Vasiljeva I.A., Konoplyannikov A.G., Erokhin V.V., Tsyb A.F., Bagdasaryan T.R., Danilenko A.A., Lepekhina L.A., Kalsina S.S., Semenkova I.V., Agaeva E.V.


Systemic transplantation of mesenchymal stem cells (MSC) is known to promote reparative process in a number of tissue damage as well as lung tissue one. It provided the basis for our study which was aimed to the effect of systemic transplantation of autologous MSC (intravenous transfusion) in complex therapy of patients with resistant pulmonary tuberculosis. Started three years ago, 27 tuberculous patients are being under our observation now; 15 patients were classified as having multidrug resistance, 12 cases - as being X-drug resistant. Bacterioexcretion and substantial pulmonary cavitation were observed in every patient, most of them (n=17) having chronic tuberculosis of 13 months to 5 years duration, the rest 10 patients diagnosed 6 to 12 months before including them to the study. All these patients had previously been undertreated or unsuccessfully treated, with one patient having one lung removed. Undergone systemic transplantation of 150-200 million of cultured MSCs derived from autlogous bone marrow the patients continued conservative therapy started before. 16 patients have been under observation for 1.5 -2 years and longer after transplantation, the rest 11 patients observed not less than 6 months. MSC- introduction gave positive clinical effects in all 27 cases: somatic status improvement, dyspnoea reduction, relieving of weakness and indisposition, putting on weight (24 cases). 3-4 months after transplantation bacterioexcretion was noticed to stop in 20 patients, in 11 patients long-termed cavities healed later. At present 9 of 16 patients who had MSC transplantation 1.5-2 years ago can be stated to have stable remission of tuberculosis, 6 patients of this group demonstrate significant positive bacteriological and morphological dynamics. Only in one of 16 cases MSC transplantation resulted in short clinical improvement. Thus, MSC transplantation as a part of antitubercular therapy can be perspective in enhancing treatment of patients with drug-resistant tuberculosis.

Genes & Cells. 2007;2(1):77-80
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