Secreted transcription factor HOXB4 promotes human ESCs endothelial lineage

M. A. Lagarkova; Лагарькова М. А.; I. A. Mufasalov; Муфазалов И. А.; P. Yu. Voltchkov; Волчков П. Ю.; S. L. Kiselyev; Киселев С. Л.

Secreted transcription factor HOXB4 promotes human ESCs endothelial lineage

Authors: Lagarkova M.A.¹, Mufasalov I.A.², Voltchkov P.Y.², Kiselyev S.L.¹
Affiliations:
1. Vavilov Institute of General Genetics, RAS
2. Institute of Gene Biology, RAS
Issue: Vol 2, No 1 (2007)
Pages: 56-59
Section: Original Study Articles
URL: https://genescells.ru/2313-1829/article/view/183008

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Abstract

The effect of the transcription factor HOXB4 on differentiation abilities of human ESCs to endothelial lineage was the aim of the present study. HOXB4 is a multifunctional transcription factor inducing or inhibiting apoptosis, promoting or repressing cell proliferation and differentiation. HOXB4 plays an important role in development and hematopoesis. OP9 stromal cells or OP9hoxb4 cells secreting transcription factor HOXB4-TAT were co-cultured with human embryonic stem cells to induce endothelial differentiation. OP9hoxb4 feeder cells significantly promoted timing of endothelium maturation both on the level of immunological makers expression and functionally in comparison with OP9 feeder. Thus our data indicate that secreted HOXB4-TAT largely influence controlled differentiation of human ESCs to endothelial cells.

Keywords

endothelium, differentiation, hESCs, HOXB4

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References

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