Secreted transcription factor HOXB4 promotes human ESCs endothelial lineage

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Abstract

The effect of the transcription factor HOXB4 on differentiation abilities of human ESCs to endothelial lineage was the aim of the present study. HOXB4 is a multifunctional transcription factor inducing or inhibiting apoptosis, promoting or repressing cell proliferation and differentiation. HOXB4 plays an important role in development and hematopoesis. OP9 stromal cells or OP9hoxb4 cells secreting transcription factor HOXB4-TAT were co-cultured with human embryonic stem cells to induce endothelial differentiation. OP9hoxb4 feeder cells significantly promoted timing of endothelium maturation both on the level of immunological makers expression and functionally in comparison with OP9 feeder. Thus our data indicate that secreted HOXB4-TAT largely influence controlled differentiation of human ESCs to endothelial cells.

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About the authors

M. A. Lagarkova

Vavilov Institute of General Genetics, RAS

Author for correspondence.
Email: redaktor@celltranspl.ru
Russian Federation, Moscow

I. A. Mufasalov

Institute of Gene Biology, RAS

Email: redaktor@celltranspl.ru
Russian Federation, Moscow

P. Yu. Voltchkov

Institute of Gene Biology, RAS

Email: redaktor@celltranspl.ru
Russian Federation, Moscow

S. L. Kiselyev

Vavilov Institute of General Genetics, RAS

Email: redaktor@celltranspl.ru
Russian Federation, Moscow

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