Comparison of effects of bone marrow different cells intramyocardial autotransplantation on reparation of rabbit myocardium after infarction

A. A. Matyukov; Матюков А. А.; N. V. Tsupkina; Цупкина Н. В.; T. D. Vlasov; Власов Т. Д.; V. V. Gritsenko; Гриценко В. В.; V. V. Davydenko; Давыденко В. В.; A. N. Yalfimov; Ялфимов А. Н.; G. P. Pinaev; Пинаев Г. П.

Comparison of effects of bone marrow different cells intramyocardial autotransplantation on reparation of rabbit myocardium after infarction

Authors: Matyukov A.A.¹, Tsupkina N.V.², Vlasov T.D.¹, Gritsenko V.V.¹, Davydenko V.V.¹, Yalfimov A.N.¹, Pinaev G.P.²
Affiliations:
1. Pavlov State Medical University of Saint-Petersburg
2. Institute of Cytology RAS
Issue: Vol 2, No 1 (2007)
Pages: 48-53
Section: Original Study Articles
URL: https://genescells.ru/2313-1829/article/view/182844
ID: 182844

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Abstract

The article represents the results of comparative estimation of effects of intramyocardial autotransplantation of bone marrow pluripotent mesenchymal stromal cells and nucleated cell on reparation of myocardium after infarction. Rabbits of the Chinchilla species were used in the experiments. Myocardial infarction was modeled by ligating the anterior descending branch of the left coronary artery. The results were assessed by a scar tissue area and the index of left ventricle dilatation in 12 months after cells transplantation. To evaluate an extend of myocardial infarction the cells were stained with 1, 3, 5, tripheniltetrasole chloride. There were three groups of animals. Into the affected* zone of the 1^st group animals (control, n=13) α-МЕМ medium was injected; to the animals of the 2^nd group (n=14) the culture of pluripotent mesenchymal stromal cells of bone marrow was introduced (2x10⁶), the 3-rd group animals (n=14) were given bone marrow nucleated cells (2x10⁶). It has been shown that transplantation of pluripotent mesenchymal stromal cells of bone marrow reduces a scar area and left ventricle dilatation as compared with the control. Bone marrow nucleated cells having been transplanted, the necrosis extends, scar area increases, every cardiac chamber substantially dilates.

Keywords

myocardial infarction, pluripotent mesenchymal stromal cells, nucleated cells, bone marrow, dilatation of left ventricle

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