Vol 11, No 3 (2016)

Full Issue


M.I. Arinkin - founder of codevelopment techniques research of cellular bone marrow: the 90-th anniversary of sternal punction

Bondarchuk S.V., Kovalev A.V., Bogdanov A.N.


Mikhail I. Arinkin was one of the prominent hematologist in their time. He is the author of intravital aspiration method bone marrow biopsy-sternal punction. Arinkin proposed a simple, minimally invasive diagnostic method of obtaining bone marrow. Almost 90 years of sternal punction remains a global priority and national Hematology, in particular, chair of faculty therapy. Security analysis of diagnostic significance of sternal punction. Mielogramm 1217, analyzed the results of cytology examination of bone marrow.
Genes & Cells. 2016;11(3):8-13
pages 8-13 views

Plant and animal stem cells: two sides of the same medal

Zubov D.A.


The review offers up-to-date analysis on plant stem cell biology, meristems functioning, including plant regeneration mechanisms and tumorigenesis, as well as biotechnology methods of plant tissue culture. There were also compared the plant and animal stem cells, their probable polyphyletic origin in aspect of divergent evolution of modular and unitary organisms in both plant and animal kingdoms.
Genes & Cells. 2016;11(3):14-22
pages 14-22 views

Application of tissue engineered constructs on the basis of cell sheets FOR RESTORATION OF TISSUES AND ORGANS

Dergilev K.V., Makarevich P.I., Menshikov M.Y., Parfyonova E.V.


Cell sheet technology has certain advantages over conventionally used injections as far as it facilitates cell survival and integration after delivery of cells to intended organ/tissue. It also allows to successfully replace lost or irreversibly damaged tissues with restoration of its functions including endo/paracrine activity. Application of cell sheets has gone beyond bench work and now is under clinical translation where it is successfully used for repair of cornea, cartilage, periodontal tissue, esophageal mucosa, pancreas and thyroid gland. Further advances of cell sheet technologies allow to construct pre-vascularized tissue grafts which effects are not limited to tissue repair, but also allows to restore its function via paracrine action of transplanted cells and to ensure long-lasting therapeutic effects. Genetic modification of cells used for cell sheet construction allows to utilize this technology to treat hereditary disorders, deficit of enzymes or other secreted proteins. This review focuses on recent results of therapeutic implication of cell sheets and prospects of this field which gained much attention in regenerative medicine.
Genes & Cells. 2016;11(3):23-32
pages 23-32 views

The natural and synthetic polymers of the non-lipid origin in gene delivery

Bogdanenko E.V., Zhdanov R.I.


For effective transfection of the eukaryotic cells with the complexes of non-viral gene carriers and plasmids it is necessary to run a number of obstacles so as a gene construction could enter a cellular nucleus and function there successfully and long. Chemical structure of a vector has the crucial importance for the targeted complex delivery to the desired organ. At present polymers of the non-lipid origin are more and more used for gene delivery along with the lipid vectors. In the review advantages and imperfections of some classes of these less used vectors are elucidated depending on their modifications and ratio to DNA and route of the delivery. We concluded that the significant advance in a task of obtaining the effective and safe vector for the human non-viral gene delivery has not been observed yet in spite of designing more and more novel variants of the gene carriers and the new kinds of the plasmids.
Genes & Cells. 2016;11(3):33-39
pages 33-39 views

Activity of NOTCH-signaling pathway after subtotal liver resection in rats

Elchaninov A.V., Fatkhudinov T.K., Kananykhina E.Y., Arutyunyan I.V., Makarov A.V., Knyazeva L.A., Bolshakova G.B., Sukhikh G.T.


N otch-signaling pathway is critical in the regulation of proliferation and differentiation of mammalian cells. Data on the activity of notch-signaling pathway after liver subtotal resection in rats have not been published yet. After subtotal liver resection in rat, the expression of notch1, notch2 and notch-targets genes (sox9, hes1, yap) was assessed with the polymerase chain reaction, immunohistochemistry and western blot. After subtotal liver resection in rat notch1, notch2, yap were downregulated and sox9, hes1 upregulated. Downregulation of notch1 and notch2 is a necessary condition for the activation of hepatocyte proliferation. Upregulation of notch-targets genes such as sox9 and hes1 it is a probably sign of deep dedifferentiation of hepatocytes and preparing them for proliferation than their transdifferentiation in cholangiocytes.
Genes & Cells. 2016;11(3):40-46
pages 40-46 views

The role of LIF-STAT3 signaling in maintaining self-renewal and pluripotent state and in rat cells

Vaskova E.A., Sherstyuk V.V., Zakian S.M.


Laboratory rat is one of the oldest and best studied objects of physiology and experimental medicine. However, rat pluripotent cells (embryonic and induced pluripotent stem cells) have been obtained relatively recently and remains poorly understood in terms of their transcriptome, proteome, epigenome as well as signaling cascades that maintain its self-renewal and pluripotency. In the study, the role of LIF-STAT3 cascade in maintaining self-renewal and pluripotency was investigated. It was shown that inhibition of the cascade led to cell cycle alteration, apoptotic and necrotic cell death. Additionally, RNA-seq data analysis was performed to identify transcripts level for LIF-STAT3 components. These results also suggest that LIF-STAT3 cascade plays an important role in self-renewal in rat pluripotent stem cells.
Genes & Cells. 2016;11(3):47-53
pages 47-53 views

Impact modified glial-derived neurotrophic factor (GDNF) for regeneration of epithelial and epithelial-stromal corneal defect in the experiment

Gavrilova N.A., Revischin A.V., Borzenok S.A., Komova O.J., Agammedov M.B., Tonaeva H.D., Ostrovsky D.S., Pavlova G.V.


Objective is to study the effect of modified glial-derived neurotrophic factor (GDNF) on healing of epithelial and epithelial-stromal corneal lesions in mice C57BL / 6J. After corneal damage the instillations of supernatant conditioned by HEK293 cells expressed GDNF gene construction without pre- and pro- sequences were produced. For control, a medium conditioned by not transfected cells was used. We assessed the area of corneal epithelial defect and corneal erosion rate, developing after the epithelial defect. The immunohistochemical study using antibodies against cytokeratin 5/18, c-Met, collagen IV, phospho-ERK1/2, phospho-JNK1/2, Ki67, Bcl2, GAP43, TIMR-1, TGF-p, Bax, and MMP 9 was performed. The area of corneal epithelial defect in the eyes of experimental animals within one day after damage was smaller than in the control. Frequencies of corneal erosions formed in the eyes of experimental animals after damage was observed in 30-35% and 80-85% of cases in the experimental and control groups, respectively. Immunohistochemical studies using these antibodies showed that GDNF stimulated the proliferative activity of epithelial cells and keratinocytes, contributed to active migration and adhesion of epithelial cells, had anti-apoptotic and antifibrotic effects, took an active part in the formation of stromal nerve plexus. The results indicate the hopefulness of therapeutic application of the modified GDNF after corneal injury and the need for further research to develop and test methods for the therapeutic use of drugs on the basis of this neurotrophic factor.
Genes & Cells. 2016;11(3):54-62
pages 54-62 views

Features of interaction between hematopoietic stem and tumor cells of different lines in vitro

Milkina E.V., Mischenko P.V., Zaytsev S.V., Bryukhovetskiy I.S., Dyuyzen I.V., Bryukhovetskiy A.S., Khotimchenko Y.S.


One of the promising directions of the brain tumors treatment connected using hematopoietic stem cells (HSC). The aim of this work: to study the features of the interaction between the human stem and tumor cells of different lines. The materials for the study were CD 34+-HSCs, U87 glioblastoma, lung cancer (A549), breast cancer (MCF-7). We used cell culture methods, the robotic monitoring of cellcell interactions, confocal laser microscopy, flow cytometry. It was shown that the mobility of HSCs to glioblastoma cells was higher than to cells of lung and breast cancer. The high mobility of HSCs combined with the ability to adhere to tumor cells and to share with them the contents of the cytoplasm. A direction of transport cytoplasm was depended from the tumor cell types and is a feature of the process. We believe that the described phenomenon can be one of the mechanisms of invasive growth and also indicates a high antitumor potential of HSCs.
Genes & Cells. 2016;11(3):63-71
pages 63-71 views

Pathogenetic parallels between native and bioprosthetic aortic valve calcification

Mukhamadiyarov R.A., Rutkovskaya N.V., Milto I.V., Vasyukov G.Y., Barbarash L.S.


There was a suggestion that pathological tissue mineralization is a universal, multifactorial, and cell-mediated process, regulated on genetic, biochemical and hormone levels. Structural deterioration of bioprosthetic heart valves is mainly caused by calcification of chemically modified xenogenic tissues. We performed this study with the aim to define parallels in degenerative strokes of native aortic valves and Ca-mediated primary tissue-insolvency of bioprosthetic aortic valves. We investigated calcified tissue samples of bioprosthetic aortic valves and calcified wings of aortic valves. (eight per each group). Functionally safe xenopericardiac mitral prosthetic heart valve, obtained at autopsy were included to investigate processes with prosthetic wings. Histological examination was carried out by hematoxylin and eosin and van Gieson staining on optical microscope AXIOImager A1. We observed mosaic structural alterations which characterized increasing of calcification process in all specimens. There were interactions between mineral deposits and valve tissues by direct contact with the cells and/or fibers of collagen and elastin. Cell composition in calcified native aortic valves was represented by fibroblasts, macrophages, multinucleated giant cells, lymphocytes, and neutrophils. Bioprosthetic heart valves also contained smooth muscle cells and capillaries. Results of our investigation represents that structures of calcified native and bioprosthetic heart valves are similar. This fact make us able to conclude that there are direct cells-mediated processes of biological material and xenogenic aortic valves degradation. Appearance of structurally safe cells in devitalized xenogenic prosthetic aortic valve caused by their functioning in recipient body. We suggest that the source of these cells can be the pluripotent progenitor cells, circulated in bloodstream and able to differentiate into cells of different phenotypes when they penetrated the collagen matrix of implanted prosthetic aortic valves. Based on the above analysis we can suggest that calcium degeneration of soft tissues is an universal pathologic process the basis of which have different mechanisms and the key one is cell-mediated mineralization.
Genes & Cells. 2016;11(3):72-79
pages 72-79 views

Immunohistochemical research of reaction of motoneurons of lumbar spinal cord of the mice that were in 30-day flight on the BION-M1 biosatellite on a week readaptation to conditions of Earth gravitation

Tyapkina O.V., Rezvyakov P.N., Nurullin L.F., Petrov K.A., Nikolskiy E.E., Islamov R.R.


Earlier, by an immunohistochemical method we define that after 30-day space flight in motoneurons of mice lumbar spinal cord immunoexpression of the proteins responsible for synaptic transfer of a nervous impulse and proteins of heat shock proteins decrease. In this research for an assessment of animals recovery process dynamics after space flight we studied an immunoexpression of the proteins participating in synaptic transfer of a nervous impulse (synaptophisyne, and PSD95), neurotrophic factors (a vascular endothelial factor of growth - VEGF and its receptor - Flt-1) and heat shock proteins (Hsp25 and Hsp70) in motoneurons of lumbar spinal cord of a mice after 30-day space flight on the BION-M1 biosatellite and the subsequent week readaptation to conditions of Earth gravitation. In this research by immunohistochemical method determine that after a week of animals staying in the Earth gravitation conditions the immunoexpression of synapt-ophisyne continued to decrease while the expression of PSD95, Hsp25, Hsp70 and VEGF increased in relation to the animals removed from experiment right after 30-day flight. The obtained data confirm functional plasticity of spinal cord motoneurons in the conditions of gravitation force changing. The fact, which is especially interesting, that «switches on» of neurons protective mechanisms (strengthening of heat shock proteins and neurotrophic factor expression) happens not in response to hypogravi-tation influence, but only a week after return of animals to conditions of Earth gravitation.
Genes & Cells. 2016;11(3):80-83
pages 80-83 views

Effect of modified coatings titanium implants on innate immunity cell

Plekhova l N.G., Lyapun I.N., Pustovalov E.V., Prosekova E.V., Gnedenkov S.V., Sinebryukhov S.L., Puz A.V.


The aim of the investigation was to investigate of the functional state effector inflammatory cells (macrophages, neutrophils) in contact with new anti-corrosion osteoinductive coatings deposited on titanium ВТ1-0. The architectonics of cellular surface, morphology, metabolism and production of cytokines by neutrophils and macrophages in their contact with titanium without coating, with the calcium phosphate coated on titanium deposited by plasma electrolytic oxidation (PEO) and PEO-coated with hydroxyapatite were studied. It was established that the most active cells adhered to the surface of the hydroxyapatite-coated titanium, while for titanium without and with calcium-phosphate PEO coated the number of these cells was significantly lower. The study of enzymes showed maximum of stimulation cellular metabolism during the first hour of contact with the coating, further, indicators of enzyme activity decreased in contrast to cells contacted with titanium. The most marked stimulation of the cellular antioxidant protection were detected in contact with a hydroxyapatite-coating. Meanwhile, the calcium-phosphate coating showed lowest immunostimulatory effect, as evidenced by indicators of tissue mediator production: cationic proteins, pro- and antiinflammatory cytokines. The coatings of titanium formed by PEO have a corrective effect on the functional state of innate immune cells reducing inflammation that develops at the foci of implant introducing.
Genes & Cells. 2016;11(3):87-93
pages 87-93 views

Biocompatibility and osteoplastic properties of mineral polymer composite materials based on sodium alginate, gelatin, and calcium phosphates intended for 3D-printing of the constructions for bone replacement

Karalkin P.A., Sergeeva N.S., Komlev V.S., Sviridova I.K., Kirsanova V.A., Akhmedova S.A., Shanskiy Y.D., Kuvshinova E.A., Filyushin M.M., Fedotov Y.A., Teterina A.Y., Zobkov Y.V., Barinov S.M., Kaprin A.D.


Bone extracellular matrix comprises a unique composite compound including the mineral and organic components. Therefore, use of biomimetic approach to the formation of tissue-engineered constructions for bone defects replacement based on composite materials containing biopolymers and calcium phosphates, as expected, can significantly improve their cyto-, biocompatibility and osteoplastic properties. The aim of the work was to study the structural features, biocompatibility and osteoplastic properties of 3D-constructions based on sodium alginate, gelatin, and two types of calcium phosphates (tricalcium phosphate and octacalcium phosphate) obtained by three-dimensional printing. The method of 3D-constructions fabrication comprised inkjet 3D-printing with hydrogel, consisted of alginate and gelatin with the addition of calcium phosphate granules, followed by freezing, freeze-drying and sterilization by y-irradiation. The structure of 3D-constructions, porosity and strength characteristics were evaluated. After the subcutaneous implantation in mice we investigated the biocompatibility of 3D-constructions during the period of up to 12 weeks. Also the osteoplastic properties of the constructions were estimated in vivo in a rat model of tibial defects. 3D printed constructions had irregular lamellar structure of sodium alginate with inclusions of spherical calcium phosphates granules. Addition of gelatin to the composite increased the porosity of constructs and significantly increased the compressive strength meanwhile practically had no effect on the ultimate strain value. In results of subcutaneous in vivo tests 3D printed constructions demonstrated perfect prolonged biocompatibility. The highest rate of biodegradation was noticed for implants containing octacalcium phosphate. All of the studied 3D-scaffolds had osteoconductive potential, more pronounced according to the number of examined histological parameters in those, made from sodium alginate, gelatin and octacalcium phosphate. The data showed the feasibility and prospect of using three-component mineral polymer composite materials based on alginate, gelatin and octacalcium phosphate as an “ink” for 3D printing of bone grafting constructions intended for implantation in bone defects.
Genes & Cells. 2016;11(3):94-101
pages 94-101 views

Povyshenie effektivnosti zaseleniya biodegradiruemykh matriksov stromal'nymi i epitelial'nymi kletkami pri dinamicheskom kul'tivirovanii

Lyundup A.V., Demchenko A.G., Tenchurin T.H., Krasheninnikov M.E., Klabukov I.D., Shepelev A.D., Mamagulashvili V.G., Oganesyan R.V., Orehov A.S., Chvalun S.N., Dyuzheva T.G.


Adhesion and proliferation of eucariotic cells are charac-terizated by high sensibility to the cultivation conditions, cell mediums, material surface properties, et al. The aim of this study was to improve seeding of stromal and epithelial cells on biodegradable matrices. We tested several polymers perspective for regenerative medicine: polycaprolacton, cellulose diacetate, PLGA, PLA/polycaprolacton. Electrospinning forming membranes had different porousity and fiber sizes. We developed a new method for 3D-matrix seeding with the use of rotation of scaffolds with cells. Based on optimal proliferation activity of the 3T3/NIH and MCF-7 cells we have chosen the scaffold compositions for multilayered cells seeding.
Genes & Cells. 2016;11(3):102-107
pages 102-107 views


Fadeyev F.A., Ulitko M.V., Lugovets D.V., Leontyev S.L., Sazonov S.V.


The potential of the automated cell culture system CompacT SelecT (TAP Biosystems, UK) allows using it for the large scale production of dermal fibroblasts for cell therapy. Automated maintenance and expansion of fibroblasts requires evaluation of the proper seeding density and defining the time for the achievement of the optimal confluency of cell monolayer for further passaging. Objective - to determine the optimal parameters of dermal fibroblasts passaging for development of standardized protocol for automated cell cultivation using CompacT SelecT system. Human dermal fibroblasts were isolated from donor skin samples by enzyme dissociation. Cells proliferation rate was evaluated by the calculation of proliferation index. The dependence of proliferation rate from cell monolayer density was determined using the value of average number of divisions of single cell per day. The dependence of fibroblasts proliferation rate from seeding density (from 50 to 1.2х104cells/cm2) in various periods of cultivation (3rd, 5th, 7th and 9th day) was investigated. The inverse correlation between proliferation rate and seeding density, together with dramatic decrease of proliferation rate at fibroblasts monolayer confluency of (3.5-4.5)х104 cells/cm2, was demonstrated. The optimal seeding density was evaluated as 3х103 cells/ cm2, the most suitable density of cell monolayer for further passaging was evaluated as 3.5х104 cells/cm2. In case of high seeding density (1.2х104 cells/cm2), on day 7, when the cell monolayer density reached the critical value, the cell extinction occured, followed by renewal of cell growth. This phenomenon may be explained by the selection of cells capable of replication under conditions of high density of monolayer.
Genes & Cells. 2016;11(3):108-112
pages 108-112 views

Molecular and cytogenetic features of primary myelofibrosis

Polushkina L.B., Martynkevich I.S., Shuvaev V.A., Fominykh M.S., Karyagina E.V., Savrilova A.M., Abdulkadyrov K.M.


Primary myelofibrosis is a myeloproliferative neoplasm characterized by bone marrow fibrosis and the risk of leukemic transformation. Clonal hematopoiesis underlying this pathology is caused by transformation of hematopoietic stem cells by somatic mutations of the genome which may lead to both aberrant proliferation and differentiation. The variability of the clinical course and prognosis of primary myelofibrosis is largely determined by the spectrum of molecular and cytogenetic defects detected in tumor cells. This review describes the currently known somatic mutations defined in patients with primary myelofibrosis and possible ways of their pathogenic action are discussed. Recent data of the impact of molecular and cytogenetic abnormalities in clinical features and prognosis of the disease were analyzed.
Genes & Cells. 2016;11(3):113-122
pages 113-122 views

Current state of EDTA-associated pseudothrombocytopenia, importance in the pathogenesis of polymorphism in the gene of platelet receptor for fibrinogen

Polyakov A.S., Goncharova E.V., Bologov S.G., Zhogolev D.K., Bondarchuk S.V., Noskov Y.A., Petrova O.R., Voronin S.V., Kolubaeva S.N.


EDTA-associated pseudothrombocytopenia a laboratory phenomen associated with the widespread introduction into laboratory practice of hematology analyzers and standardized methods for blood samples preserving. High frequency pseudothrombocytopenia among healthy individuals (20%) and among patients with various pathological conditions (50%), and lack of control of the results, can cause a large amount of laboratory and diagnostic errors. Currently there is no reliable data on the clinical value pseudothrombocytopenia. Identify EDTA-pseudothrombocytopenia based on a comparison of the data automated hematological analysis with the results of microscopic examination platelets count or automated studies using alternative anticoagulants. Detection of somatic mutations in the platelet fibrinogen receptor gene (integrin gene polymorphism ITGB3-b: 1565 T>C) in a limited study - 63.2% (12 patients surveyed from 1 9) may serve as the beginning of a new direction in understanding the etiopathogenesis and clinical significance of pseudothrombocytopenia.
Genes & Cells. 2016;11(3):123-128
pages 123-128 views

Cellular blood composition: hemogram healthy individuals in Saint Petersburg

Bondarchuk S.V., Tyrenko V.V., Mihaleva M.A., Yurkin A.K.


Presents the results of a population-based study of the numerical values of the blood of the adult male population of St. Petersburg. Studied the frequency distribution of the major indicators of the investigated cohort hemogram for 7 years. Revealed a significant variability in cellular composition. Found that interval distribution of the number of leukocytes, hemoglobin, eosinophils etc falls outside of the bounds of reference standards indicators. The most frequently mentioned decrease in the number of platelets, red blood cells, decrease in hemoglobin. Less frequently mentioned decrease in the level of lymphocytes, platelets level increase above normal interval distribution. Systemic blood diseases have been detected in the study group. Deviations can be caused by physiological oscillations, plasticity of the adaptive capacity of the blood. Thus, the distribution of the population significantly healthy hemogram feature. Justified the extension of the reference indicators of cellular components of blood.
Genes & Cells. 2016;11(3):129-134
pages 129-134 views

Eosinophilia: approaches to differential diagnosis of reactive and clonal eosinophilia

Bondarchuk S.V., Sevruk A.A., Semelev V.N.


The features of changes in blood and bone marrow of patients with reactive (1 group, n = 8) and clonal (2 group, n = 13)eosinophilia. Revealed correlations between cellular elements, depending on the etiology, a statistically significant difference between the two independent groups of patients in terms of cellular composition of the blood and bone marrow. A common feature of both groups was high blood eosinophil counts and eosinophilic hyperplasia of the bone marrow. The absence of the correlation of some indicators of peripheral blood and hematopoietic progenitors in the bone marrow in patients 1 and 2 groups. Compared with patients with reactive eosinophilias number of correlations between indicators of hemogram and myelogram in patients with klonal eosinophilias were significantly less. Identified weak false-positive relationship between the number of neutrophils in the blood and bone marrow's neutrophils with monoclonal eosinophilias. Correlation of eosinophils and basophils blood in patients with klonal eosinophilias has been declared negative (p>0.05). On the contrary, there was a clear correlation between the two groups of red blood cells and eosinophils and their precursors. It is suggested the possible disregulatory process occurring under the action of the cytokine environment hematopoietic cells in hematologic malignancies.
Genes & Cells. 2016;11(3):135-139
pages 135-139 views

Determining the number of Ilb / Ilia glycoprotein receptors and expression of P-selectin on the surface of platelets using flow cytometry in patients with acute myeloid leukemia

Semelev V.N., Tyrenko V.V., Nikitin V.Y., Sukhina I.A., Yurkin A.K., Tarakanova L.A., Demyanenko N.Y.


A study of the functional activity of platelets by flow cytometry is conducted in 11 patients with a diagnosis of AML are in clinical remission and 1 1 almost healthy volunteers. The functional activity of platelets was evaluated according to the dynamics of the number of glycoprotein receptors ( GP) IIb/IIIa on the platelet membrane and the percentage of platelets expressing P-selectin (CD62P) before and after induction 10 /тт ADP. The number of GP IIb/IIIa receptors on the platelet surface was evaluated by the mean fluorescence intensity. The average age of the subjects in the group of AML patients was 44,4±5,2 years in the control group, 38,5±6,8 years (p>0,05). In the group of AML patients platelet counts was 104,6±3,1 x109/L in the control group 210,5±20,8x109/L (p<0,01). After induction, increasing the number of receptors were observed in patients with AML, and they decrease in the control group. In patients with AML increase the number of receptors were observed after induction and the decrease in the control group. However, statistically significant differences (p>0,05) in the number of receptor GP IIb/IIIa before and after ADP stimulation in both groups have been identified. At the same time, there was no statistically significant difference (p<0,001) percent of CD62P expression on the platelet surface upon activation of ADP in AML patients and the control group. The percentage of CD62P expression on platelets in AML group increased average 2,1±0,23 times, and in the control group by 3,31±0,93 times. During the intergroup analysis it is not obtained statistically significant between the number of GP IIb/IIIa receptors and CD62P expression rate before and after induction of ADP in the studied groups. On the basis of obtained results it can be concluded that in this group of patients with AML functional activity of platelets does not differ from healthy volunteers.
Genes & Cells. 2016;11(3):140-143
pages 140-143 views

Immunological and clinical correlation in patients with rheumatoid arthritis associated with autoimmune thyroiditis

Dvorovkin A.E., Odin V.I., Inamova O.V., Tyrenko V.V., Toporkov M.M., Tzygan E.N.


Rheumatoid arthritis is a systemic autoimmune inflammatory disease of unknown etiology characterized by chronic inflammation of the synovial joints and progressive destruction of cartilage and bone. Serum and synovial fluid of RA patients revealed a wide range of autoantibodies. The highest value in the laboratory diagnosis of the disease serological tests is related to the determination of rheumatoid factor and antibodies to cyclic citrullinated peptide. In rheumatoid arthritis it is often combined with other autoimmune disorders, in particular - with autoimmune thyroiditis. Modern literature suggests that autoimmune thyroiditis and rheumatoid arthritis share common mechanisms of pathogenesis. The aim of the work was to determine the clinical and laboratory features of rheumatoid arthritis in association with autoimmune thyroiditis. We examined 146 patients with this pathology and different immunological profile. The study was conducted in three phases. In the first phase only the effect of the antibodies was determined by cyclic citrullinated peptide on the clinical and laboratory features of rheumatoid arthritis in combination with autoimmune thyroiditis. The second phase determined the effect of rheumatoid factor and antibodies to cyclic citrullinated peptide. In the third phase assessed the role of antibodies to cyclic citrullinated peptide and antibodies to citrullinated vimentin modified. The first phase showed a significant link between the immunological activity of the disease and disease activity indices, the second phase detected reliable correlation between rheumatoid factor presence in blood and age, on the third phase we found a link between immunological activity and joint destruction. Based on these data we can conclude that in patients with different immunological profile suffering from rheumatoid arthritis in combination with autoimmune thyroiditis, there were various pathogenic mechanisms of the disease.
Genes & Cells. 2016;11(3):144-149
pages 144-149 views

Comparison of cytogenetic damage in groups of patients with myeloproliferative neoplasms, depending on the presence or absence of V617F mutation in JAK2 gene

Kolubaeva S.N., Polyakov A.S., Kissel A.V., Myakoshina L.A., Viktorova N.A., Ushanov S.S., Titova A.A., Ivanov A.M., Noskov Y.A., Bologov S.G., Voronin S.V.


Myeloproliferative neoplasms are diseases characterized by clonal proliferation of myeloid bone marrow. At present, it describes a number of genetic markers myeloproliferative neoplasms, such as JAK2v617f. However the nature of violations in the progenitor cells is still not clear. In this study, a comparative analysis of cytogenetic damage in patients with myeloproliferative neoplasms with a mutation JAK2v617f and without this damage. The study showed that differences in the number and diversity of received clonal damage in both groups was not significant. Given the small number of patients with clonal cells, it can be assumed that cytogenetic analysis diagnostically significant.
Genes & Cells. 2016;11(3):150-152
pages 150-152 views

Clinical and laboratory features of different types of interferon therapy classic Ph-negative myeloproliferative neoplasms

Polyakov A.S., Tyrenko V.V., Noskov Y.A., Zhogolev D.K., Kovalev A.V.


Chronic myeloproliferative neoplasms are a group of disorders characterized by clonal disturbance of pluripotent bone marrow stem cells, leading to an excessive increase in hematopoietic cells that retain the ability to differentiate. Modern screening techniques have made changes to the current view of the epidemiology of myeloproliferative neoplasms. Recently, more and more researches are found with the median age below 50 years, and sometimes less than 40 years. This circumstance determines the need to improve therapy with the use of interferon-а. A new stage in the treatment of myeloproliferative neoplasms is the use of pegylated forms of interferon. A feature of this drug is more uniform release of interferon from copolymer combinations that results in a more effective action of the basic substance, reduced maximum weekly dose of the drug, reduce the severity of side effects, better tolerability, as well as better control of the disease progression. At the moment, even the proportion of patients receiving conventional interferon-а is very small. Therapy with pegylated interferon insufficiently developed. There are only a number of retrospective studies in recent years, indicating the usefulness of pegylated interferon to control the disease and improve the prognosis. According to the work pegylated interferon therapy is the most modern and effective approach to the treatment of chronic myeloproliferative neoplasms. We have studied the possibility of using of various forms of interferon-а for the treatment of myeloproliferative neoplasms.
Genes & Cells. 2016;11(3):153-161
pages 153-161 views

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