Activity of NOTCH-signaling pathway after subtotal liver resection in rats


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Abstract

N otch-signaling pathway is critical in the regulation of proliferation and differentiation of mammalian cells. Data on the activity of notch-signaling pathway after liver subtotal resection in rats have not been published yet. After subtotal liver resection in rat, the expression of notch1, notch2 and notch-targets genes (sox9, hes1, yap) was assessed with the polymerase chain reaction, immunohistochemistry and western blot. After subtotal liver resection in rat notch1, notch2, yap were downregulated and sox9, hes1 upregulated. Downregulation of notch1 and notch2 is a necessary condition for the activation of hepatocyte proliferation. Upregulation of notch-targets genes such as sox9 and hes1 it is a probably sign of deep dedifferentiation of hepatocytes and preparing them for proliferation than their transdifferentiation in cholangiocytes.

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About the authors

A. V Elchaninov

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology

Moscow, Russia

T. Kh Fatkhudinov

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology

Moscow, Russia

E. Y Kananykhina

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology

Moscow, Russia

I. V Arutyunyan

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology

Moscow, Russia

A. V Makarov

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology

Moscow, Russia

L. A Knyazeva

N.I. Pirogov Russian National Research Medical University

Moscow, Russia

G. B Bolshakova

Scientific Research Institute of Human Morphology

Moscow, Russia

G. T Sukhikh

Research Center for Obstetrics, Gynecology and Perinatology

Moscow, Russia

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