The role of LIF-STAT3 signaling in maintaining self-renewal and pluripotent state and in rat cells



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Abstract

Laboratory rat is one of the oldest and best studied objects of physiology and experimental medicine. However, rat pluripotent cells (embryonic and induced pluripotent stem cells) have been obtained relatively recently and remains poorly understood in terms of their transcriptome, proteome, epigenome as well as signaling cascades that maintain its self-renewal and pluripotency. In the study, the role of LIF-STAT3 cascade in maintaining self-renewal and pluripotency was investigated. It was shown that inhibition of the cascade led to cell cycle alteration, apoptotic and necrotic cell death. Additionally, RNA-seq data analysis was performed to identify transcripts level for LIF-STAT3 components. These results also suggest that LIF-STAT3 cascade plays an important role in self-renewal in rat pluripotent stem cells.

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About the authors

E. A Vaskova

Federal Research Center Institute of Cytology and Genetics, the SB of RAS; E.N. Meshalkin Novosibirsk State Research Institute of Circulation Pathology; Institute of Chemical Biology and Fundamental Medicine

Novosibirsk, Russia

V. V Sherstyuk

Federal Research Center Institute of Cytology and Genetics, the SB of RAS; E.N. Meshalkin Novosibirsk State Research Institute of Circulation Pathology; Institute of Chemical Biology and Fundamental Medicine

Novosibirsk, Russia

S. M Zakian

Federal Research Center Institute of Cytology and Genetics, the SB of RAS; E.N. Meshalkin Novosibirsk State Research Institute of Circulation Pathology; Institute of Chemical Biology and Fundamental Medicine; Novosibirsk State University

Novosibirsk, Russia

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