Effect of modified coatings titanium implants on innate immunity cell



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Abstract

The aim of the investigation was to investigate of the functional state effector inflammatory cells (macrophages, neutrophils) in contact with new anti-corrosion osteoinductive coatings deposited on titanium ВТ1-0. The architectonics of cellular surface, morphology, metabolism and production of cytokines by neutrophils and macrophages in their contact with titanium without coating, with the calcium phosphate coated on titanium deposited by plasma electrolytic oxidation (PEO) and PEO-coated with hydroxyapatite were studied. It was established that the most active cells adhered to the surface of the hydroxyapatite-coated titanium, while for titanium without and with calcium-phosphate PEO coated the number of these cells was significantly lower. The study of enzymes showed maximum of stimulation cellular metabolism during the first hour of contact with the coating, further, indicators of enzyme activity decreased in contrast to cells contacted with titanium. The most marked stimulation of the cellular antioxidant protection were detected in contact with a hydroxyapatite-coating. Meanwhile, the calcium-phosphate coating showed lowest immunostimulatory effect, as evidenced by indicators of tissue mediator production: cationic proteins, pro- and antiinflammatory cytokines. The coatings of titanium formed by PEO have a corrective effect on the functional state of innate immune cells reducing inflammation that develops at the foci of implant introducing.

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About the authors

N. G Plekhova l

Pacific State Medical University

Vladivostok, Russia

I. N Lyapun

G.P. Somov Research Institute of Epidemiology and Microbiology of RAS

Vladivostok, Russia

E. V Pustovalov

Far Eastern Federal University

Vladivostok, Russia

E. V Prosekova

Pacific State Medical University

Vladivostok, Russia

S. V Gnedenkov

Institute of Chemistry of the Far-Eastern Branch of RAS

Vladivostok, Russia

S. L Sinebryukhov

Institute of Chemistry of the Far-Eastern Branch of RAS

Vladivostok, Russia

A. V Puz

Institute of Chemistry of the Far-Eastern Branch of RAS

Vladivostok, Russia

References

  1. Попков А.В. Биосовместимые имплантаты в травматологии и ортопедии. Гений ортопедии 2014; 94-9.
  2. Hamilton R.F., Wu N., Xiang C. et al. Synthesis, characterization, and bioactivity of carboxylic acid-functionalized titanium dioxide nanobelts. Part. Fibre. Toxicol. 2014; 11: 43.
  3. Van Hove R.P., Sierevelt I.N., Van Royen B.J. et al. Titanium-nitride coating of orthopaedic implants: a review of the literature. Biomed. Res. Int. 2015; 9: 485975.
  4. Ляхов Н.З., редактор. Биокомпозиты на основе кальций-фос-фатных покрытий, наноструктурных и ультрамелкодисперсных биоинертных металлов, их биосовместимость и биодеградация. Томск: изд-во ТГУ; 2014.
  5. Montanaro L., Testoni F., Poggi A. et al. Emerging pathogenetic mechanisms of the implant-related osteomyelitis by Staphylococcus aureus. Int. J. Artif. Organs 2011; 34(9): 781-8.
  6. Pierre C.A., Chan M., Iwakura Y. et al. Periprosthetic osteolysis: characterizing the innate immune response to titanium wear-particles. J. Orthop. Res. 2010; 28(11): 1418-24.
  7. Ахтямов И.Ф., Шакирова Ф.В., Гатина Э.Б. и др. Морфологическое исследование местного действия имплантата с твердой поверхностью при индуцированной травме. Журн. Клин. Эксперим. Ортоп. им. Г.А. Илизарова 2015; 1: 65-70.
  8. Yang H.W., Lin M.H., Xu Y.Z. et al. Osteogenesis of bone marrow mesenchymal stem cells on strontium-substituted nanohydroxyapatite coated roughened titanium surfaces. Int. J. Clin. Exp. Med. 2015; 8(1): 257-64.
  9. Wu K., Xu J., Liu M. et al. Induction of osteogenic differentiation of stem cells via a lyophilized microRNA reverse transfection formulation on a tissue culture plate. Int. J. Nanomedicine 2013; 8: 1595-607.
  10. Hove R.P., Sierevelt I.N., Royen B.J. et al. Titanium-nitride coating of orthopaedic implants: a review of the literature. Biomed. Res. Int. 2015; 2015: 485975.
  11. Thomas P., Iglhaut G., Wollenberg A. et al. Allergy or tolerance: reduced inflammatory cytokine response and concomitant IL-10 production of lymphocytes and monocytes in symptom-free titanium dental implant patients. Biomed. Res. Int. 2013; 2013: 539834.
  12. Lee H.G., Hsu A., Goto H. et al. Aggravation of inflammatory response by costimulation with titanium particles and mechanical perturbations in osteoblast- and macrophage-like cells. Am. J. Physiol. Cell. Physiol. 2013; 304(5): 431-9.
  13. Moura C.C., Zanetta-Barbosa D., Dechichi P. et al. Effects of titanium surfaces on the developmental profile of monocytes/ macrophages. Braz. Dent. J. 2014; 25(2): 96-103.
  14. Kim H.D., Kim K.S., Ki S.C. et al. Electron microprobe analysis and tissue reaction around titanium alloy spinal implants. Asian Spine J. 2007; 1(1): 1-7.
  15. Tecchio C., Micheletti A., Cassatella M.A. Neutrophil-derived cytokines: facts beyond expression. Front. Immunol. 2014; 5: 508.
  16. Jhunjhunwala S., Aresta-DaSilva S., Tang K. et al. Neutrophil responses to sterile implant materials. PLoS One 2015; 10(9): e0137550.
  17. Boersema G.S., Grotenhuis N., Bayon Y. et al. The effect of biomaterials used for tissue regeneration purposes on polarization of macrophages. Biores. Open Access 2016; 5 (1): 6-14.
  18. Holt D.J., Chamberlain L.M., Grainger D.W. Cell-cell signaling in co-cultures of macrophages and fibroblasts. Biomaterials 2010; 31(36): 9382-94.
  19. Ляпун И.Н., Плехова Н.Г., Сомова Л.М. и др. Функциональная активность нейтрофилов, зараженных РНК-содержащими вирусами. ТМЖ 2012; 1: 93-6
  20. Park K.R., Bryers J.D. Effect of macrophage classical (M1) activation on implant-adherent macrophage interactions with Staphylococcus epidermidis: A murine in vitro model system. J. Biomed. Mater. Res. A 2012; 100(8): 2045-53.
  21. Masoud R., Bizouarn T., Trepout S. et al. Titanium Dioxide Nanoparticles Increase Superoxide Anion Production by Acting on NADPH Oxidase. PLoS One 2015; 10(12): e0144829.
  22. Chen X., Li H.S., Yin Y. et al. Macrophage proinflammatory response to the titanium alloy equipment in dental implantation. Genet. Mol. Res. 2015; 14(3): 9155-62.

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