The influence of P2-receptors on the cytotoxic effect of cisplatine in squamous cell uterine cervix cancer in vitro
- Authors: Raginov I.S1,2, Muhamedyarova G.K1, Panov A.V1
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Affiliations:
- Kazan State Medicale University
- Kazan (Volga region) Federal University
- Issue: Vol 9, No 3 (2014)
- Pages: 240-241
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/120330
- DOI: https://doi.org/10.23868/gc120330
- ID: 120330
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Abstract
To investigate the influence of purinergic signalling in squamous cell uterine cervix cancer in vitro, the effect of combining ATP (nonselective agonist P2-receptor) with the nonselective P2 receptor antagonists PPADS (P2X-receptors), RB2 (P2Y-receptors) and suramin (both P2Y-receptors and P2X-receptors) and the chemotherapeutic drug cisplatin was assessed. ATP effectively reduces up to twofold the growth of uterine cervix cancer cells. RB2 promoted survival of tumor cell by 52,1% compared to the control. Combinations of the cisplatin + ATP demonstrated a strong sensitivity at 2,7 fold. However, this effect of ATP was reversed in the presence of RB2 by 41%. This highlights the potential use of selective P2Y receptor agonists in the treatment of squamous cell uterine cervix cancer.
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About the authors
I. S Raginov
Kazan State Medicale University; Kazan (Volga region) Federal University
G. K Muhamedyarova
Kazan State Medicale University
A. V Panov
Kazan State Medicale University
References
- Idzko M., Ferrari D., Eltzschig H. Nucleotide signalling during inflammation. Nature 2014; 509: 310-5.
- Мингалеева Р.Н., Соловьева В.В., Блатт Н.Л. и др. Применение культур клеток и тканей для скрининга противоопухолевых препаратов in vitro // Клеточная трансплантология и тканевая инженерия 2013; 8(2): 20-8.
- Shabbir M., Ryten M., Thompson C. et al. Characterization of calcium-independent purinergic receptor-mediated apoptosis in hormone-refractory prostate cancer. BJU Int. 2008; 101(3): 352-9.
- White N., Knight G., Butler P. et al. An in vivo model of melanoma: treatment with ATP. Purinergic Signal 2009; 5(3): 32733.
- Ryu J., Jantaratnotai N., Serrano-Perez M. et al. Block of purinergic P2X7R inhibits tumor growth in a C6 glioma brain tumor animal model. J Neuropathol Exp. Neurol. 2011; 70(1): 13-22.
- Li X., Qi X., Zhou L. et al. P2X(7) receptor expression is decreased in epithelial cancer cells of ectodermal, uro-genital sinus, and distal paramesonephric duct origin. Purinergic Signal 2009; 5(3): 351-68.
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