Pharmacotherapy effect on genetic diversity of intestinal biocenosis



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Abstract

Diarrhea is one of the most frequent adverse events of H. pylori eradication therapy due to a violation of the intestinal microflora The aim of the study was to assess intestinal microflora content in H. pylori-positive and H. pylori-negative patients, as well as the effect of eradication therapy on its qualitative and quantitative composition 78 stool samples were used for analysis: 34 samples from H. pylori-positive patients before eradication therapy, 34 - from the same patients after completion of eradication therapy, 10 samples from H. pylori-negative patients (control group) A total deoxyribonucleic acid (DNA) was isolated from the stool samples by phenol extraction method, a nucleotide sequence of the isolated DNA was established by shotgun sequencing method Composition of intestinal microflora community was evaluated based on the number of species, the qualitative composition and diversity Shannon index. Statistical analysis and visualization of the analysis results were carried out in R medium Bacteroides, Prevotella, Eubacterium, Roseburia, Faecalibacterium and Clostridium were predominant genus in all groups of faecal samples, however, representation variability of the prevailing phyls (Bacteroides and Firmicutes) was lower in the control samples than in H. pylori-positive patients before and after eradication therapy Eradication therapy resulted in reduction of the Coprococcus, Bifidobacterium, Collinsella genera representation and increase in the number of Clostridium, Bacteroides, Coprobacillus and Flavonifractor. In about half of the patients eradication therapy leads to a decrease of both the number of species and the Shannon index, which indicates a decrease in the overall diversity and, consequently, reduction of the stability of community, with a possible predominance of individual species. These results suggest that changes in the intestinal microflora composition after H. pylori eradication therapy are individual and caused by initial condition of the intestinal microbiota with a predominance of its particular representatives

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About the authors

D. D Safina

Kazan (Volga region) Federal University

S. R Abdulkhakov

Kazan (Volga region) Federal University; Kazan State Medical University

Email: sayarabdul@yandex.ru

T. V Grigoryeva

Kazan (Volga region) Federal University

M. I Markelova

Kazan (Volga region) Federal University

R. K Ismagilova

Kazan (Volga region) Federal University

D. R Khusnutdinova

Kazan (Volga region) Federal University

S. Yu Malanin

Kazan (Volga region) Federal University

A. V Laikov

Kazan (Volga region) Federal University

M. N Siniagina

Kazan (Volga region) Federal University

R. A Abdulkhakov

Kazan State Medical University

V. M Chernov

Kazan (Volga region) Federal University

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