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Vol 10, No 4 (2015)

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Open Access Open Access
Restricted Access Access granted
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Peripheric disfunction as one of the mechanisms of pathogenesis of neurodegenerative disesases

Mukhamedyarov M.A., Martinov A.V., Petukhova E.O., Grigoryev P.N., Eshpay R.A., Rizvanov A.A., Zefirov A.L.


Neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, etc. affect 20-25% of elderly population, making this one of the main problems of modern society. Data accumulating during recent years certify that neurodegenerative diseases are related to disorders of not only structures on central nervous system, but of peripheral tissues and organs as well. In particular, dysfunction of peripheral excitable structures of neuromuscular and cardiovascular systems was established. These disturbances can play significant role in pathogenesis of neurodegenerative diseases, contributing to development of pathological processes underlying disability and death of patients (skeletal muscle atrophy and paralysis, myocardium infarction, etc.). Pathology of neuromuscular and cardiovascular systems in neurodegenerative diseases is not just “reflection” of degenerative changes in central nervous system, but particular, virtually unexplored aspect of pathogenesis of these maladies Peripheral dysfunctions in neurodegenerative diseases can be primary or amplifying degenerative processes in central nervous system, which further increases their importance in the development of the disease . In this paper, we conduct a detailed review of available literature and our own data on the dysfunction of the peripheral excitable structures in neurodegenerative diseases, and the role of these disorders in pathogenesis and clinical course of these pathologies
Genes & Cells. 2015;10(4):8-14
pages 8-14 views

Etiopathogenesis and principles of myasthenia gravis treatment

Kharlamova A.D., Petrov K.A., Zueva I.V., Nikolsky E.E.


The review addresses issues of etiological studies of synaptic defects underlying severe immunological disease of neuromuscular system myasthenia gravis. Current concepts on molecular mechanisms of muscle weakness accompanying this disease are considered, development of new algorithms of myasthenia gravis treatment is discussed
Genes & Cells. 2015;10(4):15-19
pages 15-19 views

Current ideas about the a-cells population and their role in the pathogenesis of diabetes mellitus

Plushkina A.S., Kaligin M.S.


Diabetes mellitus is a global problem of modern medicine All over the world scientists are study this disease in order to find new and effective therapies Numerous studies in recent years show very important role of glucagon, a hormone produced by α-cells of pancreas, in the pathogenesis of hyperglycemia in diabetes mellitus. The review analyzed current ideas about the features of the formation and differentiation of α- and β-cells of the pancreas. Particular attention is given to α-cells population and their role in the development of hyperglycemia in diabetes mellitus.
Genes & Cells. 2015;10(4):20-22
pages 20-22 views

Hepatic stellate cells - regional stem cells of the liver or a component of microenvironment?

Shafigullina A.K., Gumerova A.A., Kiassov A.P.


For years, it is debated about the nature and localization of stem cell of the liver. In recent years, a particular interest is paid to hepatic stellate cells According to the conducted researches, these cells are actively involved in restoring of hepatocytes population by different liver damages and they have a number of properties specific to stem cells. It should be noted that hepatic stellate cells are able to maintain viability in culture and differentiate into hepatocyte direction under certain conditions in vitro. Interestingly, hepatic stellate cells may themselves create such conditions for progenitor cells in vivo, as well as in vitro This is achieved thanks to the hepatic stellate cells secretion of the extracellular matrix proteins, a complex of growth factors and establishment of direct intercellular contacts. Stellate cells, localized in perisinusoidal space, are also influenced by the surrounding hepatocytes and endothelial cells Thus, perisinusoidal space is a kind of dynamic system, in which hepatocytes and endothelial cells determine the “resting” state of stellate cells, and the latter, if necessary, can be activated and participate in restoration of the liver cell populations. Based on these data, the researchers suggest that the hepatic perisinusoidal space - a niche of hepatic stellate cells, regional stem cells of the liver
Genes & Cells. 2015;10(4):23-28
pages 23-28 views

Effect of gelatin cryogel on proliferation and synthetic activity of fibroblasts in excision wound model

Yergeshov A.A., Siraeva Z.Y., Kazakova R.R., Mullin R.I., Davliev D.M., Zakirova A.A., Salikhova T.I., Kuznetsova E.V., Luong D.T., Savina I.N., Abdullin T.I.


We prepared an experimental sample of gelatin based cryogel membrane with pore size of 50-150 μm. Confocal microscopy and LDH assay showed that the cryogel macroporous structure promotes migration and proliferation of human skin fibroblasts within the matrix in vitro. To assess in vivo effect of the cryogel an excision wound model in rats was tested The cryogel significantly increases the number of fibroblasts as well as the density and order of produced collagen fibers in the dermis to day 7 of the wound healing process. The results suggest the stimulating effect of the gelatin cryogel on fibroblasts activity and demonstrate its potential for skin regeneration
Genes & Cells. 2015;10(4):29-33
pages 29-33 views

The morphological and phenotypic characteristics of microglia at different stages of cultivation and transplantation in the area of spinal cord injury in rats

Zhuravleva M.N., Mukhamedshina Y.O., Arkhipova S.S., Sanatova E.R., Rizvanov A.A.


The morphological and phenotypic characteristics of microglia were carried out immediately after isolation from the cerebral cortex of newborn rats, after 24 hours and 2 weeks of cultivation in vitro. It was shown that these cells expressed markers typical for microglia such as Iba1, CD68, CD11b/c, CD45 and Nestin throughout the cultivation period. Microglia had an amoeboid shape initially, but after 2 weeks branched forms of microglia appeared as well Freshly isolated microglia transduced with recombinant lentivirus LV-EGFP was transplanted to the site of spinal cord injury in rats. Transplanted cells survived in acute phase of injury for at least 14 days and expressed reporter EGFP
Genes & Cells. 2015;10(4):34-39
pages 34-39 views

Analysis of Bj fibroblasts mitochondrial respiratory chain function under glucose starvation and exposure to different doses of rotenone: Implications for neurogenerative diseases

Ivanova V.V., Starostina I.G., Martynova E.V., Pereira S.P., Oliveira P.J., Rizvanov A.A.


Certain neurodegenerative diseases, such as Parkinson and Alzheimer, are characterizing by an impairment in mitochondrial function and biogenesis, which may lead to pathological changes in the central nervous system. From this putative link stems a growing interest in changes to the mitochondrial electron transport chain and the ensuing energy dysfunction in neuronal cells and connective tissue cells under normal and pathological conditions Fibroblasts involved in the formation of microenvironments of different types of specialized cells from the nervous system and their dysfunction may contribute to the pathogenesis of disease as well. In this regard, we have obtained stressful conditions of human dermal fibroblasts approximating of the pathological phenotype observed in Parkinson's disease. Was studied the expression and activity of the protein complexes of the mitochondria respiratory chain including translocase TOM20 under inhibition of NADH dehydrogenase and maintaining of oxidative stress
Genes & Cells. 2015;10(4):40-46
pages 40-46 views

Platelet activation and apoptosis induced by pathogenic immune complexes containing platelet factor 4

Nevzorova T.A., Mordakhanova E.R., Andrianova I.A., Litvinov R.I.


A severe complication of heparin therapy is heparin-induced thrombocytopenia, which manifests as thrombophilia, including diffuse microthrombosis One of the main triggers of thrombosis are immune complexes formed by antibodies against platelet factor 4 and heparin , followed by massive activation of platelets probably under the influence of these pathogenic immune complexes The mechanisms of platelet impairment by the antigen-antibody complexes are largely unknown. In this work direct effects of the immune complexes formed by recombinant platelet factor 4 and a pathogenic anti-platelet factor 4 monoclonal antibody on isolated human platelets in vitro were investigated. Morphological and biochemical changes in platelets were studied by scanning electron microscopy and flow cytometry It is shown that the pathogenic immune complexes cause platelet activation including formation of pseudopods and platelet aggregation In addition, an increase of phosphatidylserine exposure on the platelet surface and a decrease in mitochondrial membrane potential were observed accompanied by formation of platelet-derived microparticles bearing phosphatidylserine The results indicate that the platelet factor 4-containing pathogenic immune complexes induce platelet activation and apoptosis, which can be an important part of the pathogenesis of thrombocytopenia and microthrombosis complicating heparin administration
Genes & Cells. 2015;10(4):47-53
pages 47-53 views

Stimulation of neurogenesis at hippocampus in Alzheimer's disease

Petukhova E.O., Mukhamedshina Y.O., Vasilieva O.Y., Aksenova L.Y., Solovyeva V.V., Garanina E.E., Rizvanov A.A., Zefirov A.L., Islamov R.R., Mukhamedyarov M.A.


Alzheimer's disease is a chronic neurodegenerative disease, which is characterized by specific pathomorphological changes in brain including hippocampus One of prospective direction in development of approaches for treatment of Alzheimer's disease is use of gene-cell technologies In present work we studied the possibility to stimulate neurogenesis at hippocampus of APP/PS1 transgenic mice by transplantation of umbilical cord blood mononuclear cells transduced with adenoviral vector overexpressing recombinant glial neurotrophic factor (GDNF). Effectiveness of therapy was evaluated basing on immunoexpression of stem and progenitor cells (using antibodies against doublecortin and nestin) in different hippocampal areas Analysis of obtained data showed that transplantation of umbilical cord blood mononuclear cells overexpressing glial neurotrophic factor or enhanced green fluorescent protein stimulates processes of neurogenesis at hippocampus of transgenic mice with Alzheimer's disease model, certifying high therapeutic potential of these gene-cell constructs
Genes & Cells. 2015;10(4):54-59
pages 54-59 views

Action of NMDA receptors agonists on amplitude of miniature endplate potentials in PRiMA and ColQ genes knockout mice

Proskurina S.E., Petrov K.A., Kharlamova A.D., Krejci E., Nikolsky E.E.


Previously it was shown that activation of NMDA receptors at neuromuscular junction can enhance nitric oxide (NO) production. NO, in turn, able to inhibit synaptic acetylcholinesterase activity and increases amplitude of synaptic responses In this study we tested a hypothesis that molecular form of acetylcholinesterase anchored in plasma membrane could be more sensitive to endogenous NO inhibition than molecular form of acetylcholinesterase located on basal lamina Experiments were performed on extensor digitorum longus of (-/-) PRiMA, (-/-) ColQ and wild type mice Miniature endplate potentials were recorded using standard microelectrode technique. After application of NMDA receptors agonists (glutamate and glycine) amplitude and decay time of miniature endplate potentials did not change in synapses of PRiMA or ColQ knock-out mice. Obtained results show that nitric oxide production owing to the activation of enzyme NO-synthase, which is initiated by glutamate mediated excitation of synaptic NMDA receptors, does not affect selectively one of molecular forms of acetylcholinesterase
Genes & Cells. 2015;10(4):60-62
pages 60-62 views

Nanocomposite material for stomatology restovration particles cytotoxicity: an in vitro study

Saleeva G.T., Gimaletdinova A.M., Tarasova E.Y., Rozhina E.V., Naumenko E.A., Fakhrullin R.F., Islamov R.R., Saleev R.A.


Nanocomposite material applied in dental practice treatment is subjected to mechanical abrasion with the release of material’s fine particles, including nanoparticles, which are able to produce a specific biological effect on patient body’s tissues and cells. I the present research we studied the cytotoxicity of 3MTMESPETMFiltekTMUltimate nanocomposite particles at concentration ranging from 10 to 0. 009 mg/ml employing human lung carcinoma cell culture (A549). Linear dependence of nanocomposite particles’ action on survival and functional activity of the cells was shown. The crucial concentration of cytotoxicity should be considered as 0. 313 mg/ml. At 0.156 mg/ml or below this concentration the tested substance had no toxic effect on A549 cells Considering the possible negative effect of nanocomposite particles released during the material abrasion on cells we suggest limiting application of the studied nanocomposite material on the surfaces subjected to intensive mechanical impact leading to high abrasion
Genes & Cells. 2015;10(4):63-67
pages 63-67 views

Expression of myogenesis genes by gingiva derived cells

Samchuk D.P., Lukyanova E.N., Eremin I.I., Zorin V.L., Zorina A.I., Grinakovskaya O.S., Korsakov I.N., Deev R.V., Gilmutdinova I.R., Lazareva N.L., Eremin P.S., Petrikina A.P., Gomzyakov A.E., Timashkov D.A., Vit’ko N.K., Kotenko K.V., Kopnin P.B., Pulin A.A.


The ability of gingiva derived mesenchymal stromal cells (MSCs) to myogenic differentiation and the possibility of their expansion in vitro revealed in our previous work, open up prospects for their use in regenerative medicine for the correction of muscle pathology. Data on changes in the expression of myogenic regulatory factors in gingiva derived MSCs can serve as confirmation of the possibility of differentiation of these cells into the myogenic direction The study was conducted on cultures of gingiva derived MSCs and skin fibroblasts. When comparing gene expression profiles of gingiva derived MSCs and skin fibroblasts significant changes have been registered for 153 genes. Of these, we selected 19 significant differentially expressed genes, for which main signal pathways were analyzed. Evidences of significant activation / inactivation of signaling pathways and key genes that have been identified for the population of human satellite cells were obtained during analysis of the genome transcriptome profiles of gingiva derived MSCs This applies to such signaling pathways as the p38 MAPK, NOTCH and other groups responsible for the proliferation and differentiation in myogenic direction, the processes of self-renewal or self-maintenance in the population of satellite cells. At the same time full match in the patterns of gene expression between the stable population of gingiva derived MSCs and human satellite cells undergoing myogenic differentiation as well as cells derived from atypical sources and differentiated in the myogenic direction was not observed The data of our experiment suggests that the gingiva derived MSCs are distinct from satellite cells and could be considered as population with plateaued development, possessing the ability to differentiate into myogenic direction due to non-canonical expression pattern of myogenic regulatory factors
Genes & Cells. 2015;10(4):68-77
pages 68-77 views

Pharmacotherapy effect on genetic diversity of intestinal biocenosis

Safina D.D., Abdulkhakov S.R., Grigoryeva T.V., Markelova M.I., Ismagilova R.K., Khusnutdinova D.R., Malanin S.Y., Laikov A.V., Siniagina M.N., Abdulkhakov R.A., Chernov V.M.


Diarrhea is one of the most frequent adverse events of H. pylori eradication therapy due to a violation of the intestinal microflora The aim of the study was to assess intestinal microflora content in H. pylori-positive and H. pylori-negative patients, as well as the effect of eradication therapy on its qualitative and quantitative composition 78 stool samples were used for analysis: 34 samples from H. pylori-positive patients before eradication therapy, 34 - from the same patients after completion of eradication therapy, 10 samples from H. pylori-negative patients (control group) A total deoxyribonucleic acid (DNA) was isolated from the stool samples by phenol extraction method, a nucleotide sequence of the isolated DNA was established by shotgun sequencing method Composition of intestinal microflora community was evaluated based on the number of species, the qualitative composition and diversity Shannon index. Statistical analysis and visualization of the analysis results were carried out in R medium Bacteroides, Prevotella, Eubacterium, Roseburia, Faecalibacterium and Clostridium were predominant genus in all groups of faecal samples, however, representation variability of the prevailing phyls (Bacteroides and Firmicutes) was lower in the control samples than in H. pylori-positive patients before and after eradication therapy Eradication therapy resulted in reduction of the Coprococcus, Bifidobacterium, Collinsella genera representation and increase in the number of Clostridium, Bacteroides, Coprobacillus and Flavonifractor. In about half of the patients eradication therapy leads to a decrease of both the number of species and the Shannon index, which indicates a decrease in the overall diversity and, consequently, reduction of the stability of community, with a possible predominance of individual species. These results suggest that changes in the intestinal microflora composition after H. pylori eradication therapy are individual and caused by initial condition of the intestinal microbiota with a predominance of its particular representatives
Genes & Cells. 2015;10(4):80-85
pages 80-85 views

Homing and survivability of genetically modified mononuclear umbilical cord blood cells after transplantation into transgenic G93A mice with amyotrophic lateral sclerosis

Safiullov Z.Z., Garanina E.E., Izmailov A.A., Garifulin R.R., Fedotova V.Y., Salafutdinov I.I., Rizvanov A.A., Islamov R.R.


To overcome the effects of neurodegeneration, as an alternative option of pharmacotherapy, the genetically modified human umbilical cord blood mononuclear cells overexpressing vascular endothelial growth factor (VEGF), glial-derived neurotrophic factor (GDNF), neuronal cell adhesion molecule (NCAM) were suggested. The migration potential and survivability of the genetically modified cells based on expression of therapeutic gene in different combinations after transplantation into G93A mice with amyotrophic lateral sclerosis model was studied The pattern of homing and survivability of the genetically modified cells in a spinal chord after different period of time after treatment was shown by immunofluorescent analysis Based on the level of survived cells and the combination of therapeutic genes the life extension of G93A mice was observed.
Genes & Cells. 2015;10(4):86-89
pages 86-89 views

Effect of tescalcin overexpression on osteogenic differentiation of human mesenchymal stem cells

Solovyeva V.V., Kolobynina K.G., Gomzikova M.O., Tazetdinova L.G., Zhuravleva M.N., Slepak V.Z., Rizvanov A.A.


Tescalcin plays an important role in the proliferation and differentiation of certain cell types. It is involved in the signaling pathways of mitogen-activated protein kinase and is reported to directly interact with subunit 4 of COP9 signalosome and Na/H exchanger NHE1. Since tescalcin is one of the factors that allow one type of progenitor cells differentiate into a variety of specialized cell types, it can be a potential molecular tool for modulation of phenotype of target cells. The ability of adipose-derived mesenchymal stem cells (ADSCs) to differentiate into osteogenic direction makes them a promising adult stem cell type for regenerative medicine and tissue engineering. Here we show that ADSCs with tescalcin overexpression had a significantly higher level of matrix mineralization compared with control ADSCs. This finding indicates that ectopic tescalcin overexpression might affect osteogenic differentiation of ADSCs.
Genes & Cells. 2015;10(4):90-93
pages 90-93 views

Direct modification of cells using polycation-stabilized silver nanoparticles

Tarasova E.Y., Naumenko E.A., Rozhina E.V., Fakhrullin R.F.


Here we report the surface functionalization of human lung carcinoma cells A549 with polyallylamine hydrochloride-stabilized silver nanoparticles. We investigated the time and dose-dependent effects of AgPAH on cells, the influence of functionalization on the viability of cells, monolayer formation and proliferative activity Safe concentration of silver nanoparticles stabilized with polyallylamine hydrochloride for A549 cells were determined The differential effects of silver nanoparticles stabilized with polyallylamine hydrochloride on cell function and organelles were revealed
Genes & Cells. 2015;10(4):94-98
pages 94-98 views

Transplantation of hepatic stellate, genetically modified genes hgf and fgf4, stimulates differentiation of hepatocytes in rats after partial hepatectomy

Titova A.A., Sharipova E.I., Pevnev G.O., Mavlikeev M.O., Shafigullina A.K., Burganova G.R., Titova M.A., Rizvanov A.A., Gumerova A.A., Kiyasov A.P.


Chronic liver diseases of different etiology are the problem of the modern medicine. Nowadays is held an active search for new methods of treatment for these diseases using stem cells and methods of gene-cell therapy. One of the cell types, thought to be progenitor cells of the liver, - hepatic stellate cells. Previously was shown the possibility of hepatic stellate cells to differentiate into the cells with hepatocytes phenotype after transplantation into the rats with partial hepatectomy. In this work we studied rat liver regeneration on the same model of liver damage after transplantation of the hepatic stellate cells, transduced with therapeutic genes of hepatocyte growth factor (HGF) and fibroblast growth factor 4 (FGF4) in combination with tracer gene of red fluorescent protein (RFP). The results of the survey demonstrated that transduction of the hepatic stellate cells with these genes facilitates engraftment of the transplanted cells and their further differentiation into hepatic direction, that was confirmed by a large number of hepatocytes, expressing tracer gene RFP, as well as a large number of α-fetoprotein positive hepatoblasts in compare to the animals, that had transplantation of cells, transduced only with tracer gene
Genes & Cells. 2015;10(4):99-102
pages 99-102 views

Role of K(ATP)-channels in the effects of hydrogen sulfide on the contractility of rat ventricular myocardium

Khaertdinov N.N., Lifanova A.S., Gizzatullin A.R., Sitdikova G.F.


The effect of sodium hydrosulfide (NaHS) - donor of hydrogen sulfide (H2S) on the force of contraction of isolated rat ventricle was studied. Application of NaHS in concentrations 1 and 10 μМ resulted in an increase and in concentrations 50, 100, 200 and 300 μМ - dose-dependent decrease of the force of contraction. Inhibition of K-channels by tetraethylammonium (15 mM) caused the increase of the amplitude of contraction and the negative inotropic effect of NaHS (200 μМ) was preserved. After the activation or inhibition of ATP-dependent K-channels by diazoxide (20 μМ) or glibenclamide (50 μМ), respectively, the negative inotropic effect of NaHS was the same as in control After preliminary application of NaHS glibenclamide (50 μМ) reversed the contraction force to yhe control values. The obtained data suppose that in the isolated rat ventricle exogenous H2S causes a reduction of the force of contraction, which is mediated by the activation of ATP-dependent K-channels
Genes & Cells. 2015;10(4):103-105
pages 103-105 views

Lymphocyte apoptosis in neonatal sepsis due to klebsiella

Khaertynov K.S., Boichuk S.V., Anokhin V.A., Ramazanov B.R., Rizvanov A.A., Khaiboullina S.F., Lubin S.A., Agapova I.V.


The apoptosis of lymphocytes was examined in 10 infants with late neonatal sepsis induced by Klebsiella pneumoniae Most of the infants (70%) were preterm. Control group consisted of 7 healthy newborns. Apoptosis analysis was examined by counting the numbers of hypodiploid cells by using a propidium iodide (DNA staining procedure and flow cytometry analysis. We observed an elevated numbers of apoptotic cells in all cases of neonatal sepsis Median of lymphocyte apoptosis was 18 1% on day 5 of cell culture, whereas in control patients it was up to 7,8%. Absolute lymphopenia was observed in 30% of cases with neonatal sepsis. The activity of lymphocyte apoptosis was not associated with quantitative baseline of C-reactive protein (Spearman R = 0,48, p = 0,18). Acute phase of neonatal sepsis induced by Klebsiella pneumonia is associated with an increased apoptosis of peripheral blood lymphocytes
Genes & Cells. 2015;10(4):106-109
pages 106-109 views

Use of stromal vascular fraction cells from adipose tissue to replace segmental defect of dog's alveolar crest: experimental case

Khairutdinova A.R., Khafizova F.A., Mirgazizov M.Z., Khafizov I.R., Zakirova E.Y., Sergeev M.A., Salafutdinov I.I., Masgutova G.A., Masgutov R.F., Rizvanov A.A., Bagmanov M.A., Mirgazizov R.M., Khafizov R.G.


The paper presents a case of using autologous stromal-vascular fraction of adipose tissue to increase bone volume in the areas of dental implantation. As the matrix for cells we used nikelid-porous titanium granules which fit into the defect area alveolar with giving required volume. Throughout the experiment conducted observation of animals, accompanied by histological studies According to the results of histological studies and observations it can be concluded that the application of the proposed method of bone plastic is advisable and highly effective in the practice of medicine.
Genes & Cells. 2015;10(4):110-113
pages 110-113 views

Influence of Hepatic Stellate Cells transduction by adenoviral vector Ad5-optHGF-optFGF-4-RFP on their phenotype in vitro and in vivo

Sharipova E.I., Titova A.A., Shafigullina A.K., Galyavieva A.R., Garanina E.E., Mavlikeev M.O., Pevnev G.O., Burganova G.R., Titova M.A., Gumerova A.A., Kiyasov A.P., Rizvanov A.A.


Nowadays gene and cell therapy methods for liver diseases treatment are being actively developed. Genetic modification of cells could be an approach that considerably increases the therapeutic potential of transplanted cells. It is assumed that the genetic manipulation, particularly obtaining and application of cells, that express and overexpress therapeutic factors, could reduce the therapeutic dose of transplanted cells and noticeably enhance therapeutic effects of these cells. However, it remains unclear, how genetic modification influences on cellular phenotype, in this case hepatic stellate cells, and what kind of therapeutic effect will give these cells after transplantation into the organism. The aim of our work was to study the phenotype of hepatic stellate cells after genetic modification by the adenoviral vector Ad5-optHGF-optFGF-4-RFP in vitro and in vivo and after subsequent transplantation into the rats with partial hepatectomy. As a result, it was confirmed that transplantation of hepatic stellate cells, transduced with therapeutic genes, has a positive influence on the process of liver regeneration while the morphology and phenotype of cells remain unchanged. So, we can make a conclusion of safety of this method for use in regenerative medicine
Genes & Cells. 2015;10(4):114-117
pages 114-117 views

Polymorphism of genes of the antioxidant system in patients with erysipelas and their role in development of the disease

Emene C.P., Kravchenko I.E., Aibatova G.I., Rizvanov A.A.


In this research we analyzed the genotype distribution of polymorphisms of genes involved in antioxidant system of erysipelas patients and healthy population in Republic of Tatarstan, Russia. On comparing genotype distribution with healthy controls, we identified that SNPs of superoxide dismutase 1 - SOD1g7958a, superoxide dismutase 2 - SOD2 T5482C (Ala16Val) and catalase - CAT C262T affected the predisposition to erysipelas infection and clinical features
Genes & Cells. 2015;10(4):118-122
pages 118-122 views

Parameters of single and summated contractions of skeletal muscles in vivo and in vitro

Eshpay R.A., Khairullin A.E., Karimova R.G., Nurieva L.R., Rizvanov A.A., Mukhamedyarov M.A., Ziganshin A.U., Grishin S.N.


It is well known that modern non-invasive methods for registration of functional activity of skeletal muscles in vivo due to technical reasons reflect amplitude and temporal parameters of muscle contractions not better than in vitro approaches The aim of present study is to register and compare parameters of single and tetanic contractions of different types of skeletal muscles of warm-blooded animals in vitro and under minimally invasive surgery in vivo. It was found that single muscle contractions in vitro and in vivo indistinguishably close to each other, whereas frequency of tetanus fusion and waveform of tetanic contractions are different.
Genes & Cells. 2015;10(4):123-126
pages 123-126 views

Experimental models for studying of skeletal muscles regeneration

Chernova O.N., Korsakov I.N., Samchuk D.P., Pulin A.A., Mavlikeev M.O., Deev R.V., Eremin I.I.


Striated muscles play an important role in the maintenance in the maintenance of locomotion, ventilation, mechanical protection, the inner organs support, a common system of energy exchange etc. Skeletal muscle tissue is exposed to various external factors which cause notable damage to skeletal tissue as a result of mechanical injury (contusion, compression, laceration), inflammation as an implication of infectious agents and autoimmune process, toxic effects of various chemical substances. Besides the external causes, genes' defects that code muscle protein components have influence on the muscles too. These defects lead to muscular dystrophies (Duchenne Becker muscular dystrophy, dysferlinopathy, calpainopathy etc.). In condition of serious injuries the cambial reserve by means of myosatellite cells and other myogenic cells usually does not provides hysto-and organotypic skeletal muscles regeneration. This fact determines development of new methods for induction of regeneration striated muscles and, in turn, requires amplification of using experimental models of muscles injury for studying regeneration of skeletal muscles The aim of this review is comparative description of experimental models applied for studying of skeletal muscles regeneration after its damage
Genes & Cells. 2015;10(4):127-140
pages 127-140 views

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