Role of autophagy in response of epithelial ovarian cancer cells to cisplatin treatment and cisplatin resistance



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Abstract

Ovarian cancer survival rate is inversely associated with the extent of tumor metastasis. One of the main treatment approaches against ovarian cancer is employment of platinum based therapies, including cisplatin. Majority of ovarian cancer patients develop cisplatin resistance. We aimed to investigate roles for macroautophagy in response of epithelial ovarian cancer cells to cisplatin, including changes in cell motility, as well as in development of cisplatin resistance. Cisplatin treatment induced autophagy in Caov-3 cells in vitro, as well as resulted in increased cell motility. Pharmacologic inhibition of autophagy by wortmannin eliminated the effect of cisplatin on cell motility. We further selected Caov-3 cells with acquired cisplatin resistance and observed elevated baseline expression of autophagy markers in the resistant cells. Our data indicate a role for autophagy in development of cisplatin resistance by the EOC cells, as well as a potential role for cisplatin-induced autophagy in ovarian tumor metastasis.

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About the authors

A. M Mazitova

Kazan (Volga region) Federal University

Iu. A Topchu

Kazan (Volga region) Federal University

L. A Mingazova

Kazan (Volga region) Federal University

E. M Biktagirova

Kazan (Volga region) Federal University

Z. I Abramova

Kazan (Volga region) Federal University

R. T Gabbasov

Kazan (Volga region) Federal University

Email: rtgabbasov@gmail.com

References

  1. Bray F., Ferlay J., Soerjomataram I. et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. A Cancer J. Clinicians 2018; 68(IV): 387-506.
  2. Reid B.M., Permuth J.B., Sellers T.A. Epidemiology of ovarian cancer: a review. Cancer Biol. & Med. 2017; 14(I): 9-32.
  3. Cortez A.J., Tudrej P., Kujava K.M. et al. Advances in ovarian cancer therapy. Cancer Chemother. Pharmacol. 2018; 81(1): 17-38.
  4. Bast R.C., Jr, Hennessy B., Mills G.B. The biology of ovarian cancer: new opportunities for translation. Nature reviews Cancer 2009; 9(6): 415-28.
  5. Freedman R.S., Deavers M., Liu J. et al. Peritoneal inflammation - A microenvironment for Epithelial Ovarian Cancer (EOC). J. Transl. Med. 2004; 2(1): 23.
  6. Рябая О.О., Егорова А.В., Степанова Е.В. Роль аутофагии в механизмах гибели опухолевых клеток. Успехи современной биологии 2015; 135(II2): 177-88. [Ryabaya O.O., Egorova A.V., Stepanova E.V. The Role of Autophagy in Mechanisms of Tumor Cell Death. Advances in Modern Biology 2015; 135(II2): 177-88].
  7. Peracchio C., Alabiso O., Valente G. et al. Involvement of autophagy in ovarian cancer: a working hypothesis. J. Ovarian Res. 2012; 5: 22.
  8. Mowers E., Sharifi M., Macleod K. Autophagy in cancer metastasis. Oncogene 2017; 36: 1619-30.
  9. Takahashi A., Kimura T., Takabatake Y. et al. Autophagy guards against cisplatin-induced acute kidney injury. Am.J. Pathol. 2012; 180: 517-25.
  10. Chen J., Zhang L., Zhou H. et al. Inhibition of autophagy promotes cisplatin-induced apoptotic cell death through Atg5 and Beclin 1 in A549 human lung cancer cells. Mol. Med. Rep. 2018; 17(V): 6859-65
  11. Fan Z., Huangfu X., Liu Z. Effect of autophagy on cisplatin caused bladder cancer cell apoptosis. Panminerva Med. 2016; 59: 1-8
  12. Bao L., Jaramillo M.C., Zhang Z. Induction of autophagy contributes to cisplatin resistance in human ovarian cancer cells. Mol. Med. Rep. 2014; 11(1): 91-8.
  13. Wang J., Wu G.S. Role of autophagy in cisplatin resistance in ovarian cancer cells. J. Biol. Chem. 2014; 289: 17163-73.
  14. Zhu J., Zheng Y., Zhang H. et al. Low concentration of chloroquine enhanced efficacy of cisplatin in the treatment of human ovarian cancer dependent on autophagy. Am.J. Transl. Res. 2017; 9(IX): 4046-58.
  15. He J., Yu J.J., Xu Q. Down regulation of ATG14 by EGR1-MIR152 sensitizes ovarian cancer cells to cisplatin-induced apoptosis by inhibiting cyto-protective autophagy. Autophagy 2015; 11(H): 373-84.
  16. Domcke S., Sinha R., Levine D.A. et al. Evaluating cell lines as tumour models by comparison of genomic profiles. Nat. Commun. 2013; 4: 2126.
  17. Klionsky D.J., Abdelmohsen K., Abe A. et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 2016; 12: 1-222.
  18. Han W., Pan H., Chen Y. et al. EGFR Tyrosine Kinase Inhibitors Activate Autophagy as a Cytoprotective Response in Human Lung Cancer Cells. PLoS One 2011; 6: 18691.
  19. Green D.R., Galluzzi L., Kroemer G. Mitochondria and the autophagy-inflammation-cell death axis in organismal aging. Science 2011; 333: 1109-12.
  20. Wu T., Liang X., Wang M.C. et al. Autophagy facilitates lung adenocarcinoma resistance to cisplatin. Drug Des. Devel. Ther. 201 5; 9: 6421-31.
  21. Chen X., Zhang L., Ding S. et al. Cisplatin combination drugs induce autophagy in HeLa cells and interact with HSA via electrostatic binding. RSC Adv. 2017; 7: 22270-9.
  22. Yang Y.P., Hu L.F., Zheng H.F. et al. Application and interpretation of current autophagy inhibitors and activators. Acta pharmacol. Sinica 2013; 34(5): 625-35.

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