Obtaining and characteristics of human induced pluripotent stem cells from skin fibroblastsof patients with neurodegenerative diseases
- Authors: Nekrasov ED1, Lebedeva OS2, Chestkov IV1, Syusina MA1, Fedotova EY.3, Lagarkova MA1, Kiselev SL4, Grivennikov IA5, Illarioshkin SN3
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Affiliations:
- N.I. Vavilov Institute of General Genetics, RAS, Moscow
- N.I. Vavilov Institute of General Genetics, RAS, MoscowMolecular Genetics Institute, RAS, Moscow
- Research Neurology Center, RAMS, Moscow
- N.I. Vavilov Institute of General Genetics, RAS, MoscowNational Research Institute «Kurchatov Institute», Moscow
- Molecular Genetics Institute, RAS, Moscow
- Issue: Vol 6, No 4 (2011)
- Pages: 82-88
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121688
- DOI: https://doi.org/10.23868/gc121688
- ID: 121688
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Abstract
to embryonic stem cells in their properties, and might be
derived from adult somatic cells. There exist two main
approaches to practical application of iPSCs technologies -
they are regenerative medicine and human diseases modeling.
Nowadays the process of iPSCs obtaining is being investigated
in many world laboratories, but there are no standards in the
iPSC obtaining technology. This work was aimed to develop a
simple, cheap and practical protocol for obtaining iPSCs and
to establish a collection of iPCS lines derived from cells of
patients with a Parkinson disease to make a cell model of this
disease. The work resulted in a development of the protocol
to produce iPSCs based on a lentiviral delivery of transgenes
into cells. iPCS lines from 3 patients with inherited Parkinson
disease forms have been obtained.
About the authors
E D Nekrasov
N.I. Vavilov Institute of General Genetics, RAS, MoscowN.I. Vavilov Institute of General Genetics, RAS, Moscow
O S Lebedeva
N.I. Vavilov Institute of General Genetics, RAS, MoscowMolecular Genetics Institute, RAS, MoscowN.I. Vavilov Institute of General Genetics, RAS, MoscowMolecular Genetics Institute, RAS, Moscow
I V Chestkov
N.I. Vavilov Institute of General Genetics, RAS, MoscowN.I. Vavilov Institute of General Genetics, RAS, Moscow
M A Syusina
N.I. Vavilov Institute of General Genetics, RAS, MoscowN.I. Vavilov Institute of General Genetics, RAS, Moscow
E Yu Fedotova
Research Neurology Center, RAMS, MoscowResearch Neurology Center, RAMS, Moscow
M A Lagarkova
N.I. Vavilov Institute of General Genetics, RAS, MoscowN.I. Vavilov Institute of General Genetics, RAS, Moscow
S L Kiselev
N.I. Vavilov Institute of General Genetics, RAS, MoscowNational Research Institute «Kurchatov Institute», MoscowN.I. Vavilov Institute of General Genetics, RAS, MoscowNational Research Institute «Kurchatov Institute», Moscow
I A Grivennikov
Molecular Genetics Institute, RAS, MoscowMolecular Genetics Institute, RAS, Moscow
S N Illarioshkin
Research Neurology Center, RAMS, MoscowResearch Neurology Center, RAMS, Moscow
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