Genes & CellsGenes & Cells2313-18292500-2562Human Stem Cells Institute12168810.23868/gc121688Obtaining and characteristics of human induced pluripotent stem cells from skin fibroblastsof patients with neurodegenerative diseasesNekrasovE DN.I. Vavilov Institute of General Genetics, RAS, Moscow-LebedevaO SN.I. Vavilov Institute of General Genetics, RAS, MoscowMolecular Genetics Institute, RAS, Moscow-ChestkovI VN.I. Vavilov Institute of General Genetics, RAS, Moscow-SyusinaM AN.I. Vavilov Institute of General Genetics, RAS, Moscow-FedotovaE YuResearch Neurology Center, RAMS, Moscow-LagarkovaM AN.I. Vavilov Institute of General Genetics, RAS, Moscow-KiselevS LN.I. Vavilov Institute of General Genetics, RAS, MoscowNational Research Institute «Kurchatov Institute», Moscow-GrivennikovI AMolecular Genetics Institute, RAS, Moscow-IllarioshkinS NResearch Neurology Center, RAMS, Moscow-N.I. Vavilov Institute of General Genetics, RAS, MoscowN.I. Vavilov Institute of General Genetics, RAS, MoscowMolecular Genetics Institute, RAS, MoscowResearch Neurology Center, RAMS, MoscowN.I. Vavilov Institute of General Genetics, RAS, MoscowNational Research Institute «Kurchatov Institute», MoscowMolecular Genetics Institute, RAS, Moscow1512201164828811012023Copyright © 2011, Eco-Vector2011Induced pluripotent stem cells (iPSCs) are analogous
to embryonic stem cells in their properties, and might be
derived from adult somatic cells. There exist two main
approaches to practical application of iPSCs technologies -
they are regenerative medicine and human diseases modeling.
Nowadays the process of iPSCs obtaining is being investigated
in many world laboratories, but there are no standards in the
iPSC obtaining technology. This work was aimed to develop a
simple, cheap and practical protocol for obtaining iPSCs and
to establish a collection of iPCS lines derived from cells of
patients with a Parkinson disease to make a cell model of this
disease. The work resulted in a development of the protocol
to produce iPSCs based on a lentiviral delivery of transgenes
into cells. iPCS lines from 3 patients with inherited Parkinson
disease forms have been obtained.[Takahashi K., Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 126(4): 663-76.][Takahashi K., Tanabe K., Ohnuki M. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007; 131(5): 861-72.][Maherali N., Hochedlinger K. Guidelines and techniques for the generation of induced pluripotent stem cells. Cell Stem Cell 2008; 3(6): 595-605.][McNeish J., Roach M., Hambor J. et al. High-throughput screening in embryonic stem cell-derived neurons identifies potentiators of alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate- type glutamate receptors. J. Biol. Chem. 2010; 285(22): 17209-17.][Woltjen K., Michael I.P., Mohseni P. et al. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 2009; 458(7239): 766-70.][Okita K., Nakagawa M., Hyenjong H., Ichisaka T., Yamanaka S. Generation of mouse induced pluripotent stem cells without viral vectors. Science 2008; 322(5903): 949-53.][Lai M.I., Wendy-Yeo W.Y., Ramasamy R. et al. Advancements in reprogramming strategies for the generation of induced pluripotent stem cells. J. Assist. Reprod. Genet. 2011; 28(4): 291-301.][Weber K., Mock U., Petrowitz B., Bartsch U., Fehse B. Lentiviral gene ontology (LeGO) vectors equipped with novel drug-selectable fluorescent proteins: new building blocks for cell marking and multigene analysis. Gene Ther. 2010; 17(4): 511-20.][Некрасов Е.Д., Лагарькова М.А., Киселев С.Л. Индуцирован- ные плюрипотентные стволовые клетки как модель для изучения болезней человека. КТТИ 2011; 6(2): 32-7.][Philonenko E.S., Shutova M.V., Chestkov I.V., Lagarkova M.A., Kiselev S.L. Current Progress and Potential Practical Application for Human Pluripotent Stem Cells. Int. Rev. Cell Mol. Biol. 2011; 292: 153-96.][Saha K., Jaenisch R. Technical challenges in using human induced pluripotent stem cells to model disease. Cell Stem Cell 2009; 5(6): 584-95.][Huangfu D., Maehr R., Guo W. et al. Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat. Biotechnol. 2008; 26(7): 795-7.][Shi Y., Desponts C., Do J.T. et al. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 2008; 3(5): 568-74.][Warren L., Manos P.D., Ahfeldt T. et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 2010; 7(5): 618-30.][Okita K., Matsumura Y., Sato Y. et al. A more efficient method to generate integration-free human iPS cells. Nat. Methods 2011; 8(5): 409-12.]