Personalized cell therapy in ophthalmology. I. Method for obtaining and phenotype of autologous product
- Authors: Avetisov SE1, Subbot AM2, Antochin AI2, Kasparova EA1, Kasparov AA1, Pavliuk AS2
-
Affiliations:
- State Research Institute of Eye Diseases RAMS
- The Russian State Medical University
- Issue: Vol 6, No 2 (2011)
- Pages: 38-42
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121656
- DOI: https://doi.org/10.23868/gc121656
- ID: 121656
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Abstract
autologous leucocytes from peripheral blood for corneal
diseases treatment is developed. Method allows us to obtain
a product with sufficient content of viable nucleated cells (up
to 3106 cell/ml). Cellular composition of the product was
determined. Mononuclear fraction decreased in 1,3 times and
granulocyte portion is increased. Phenotype of lymphocytes
in the cell product is the same as in peripheral blood, with
selective loss of B-lymphocytes. The presence of CD14+/34low
cells, which are considered to be progenitors, is defined in the
product. Using of cell product, obtained by designed technique,
may be promising in clinic, because of it minimal traumatism
for the patient and simplicity.
About the authors
S E Avetisov
State Research Institute of Eye Diseases RAMSState Research Institute of Eye Diseases RAMS
A M Subbot
The Russian State Medical UniversityThe Russian State Medical University
A I Antochin
The Russian State Medical UniversityThe Russian State Medical University
E A Kasparova
State Research Institute of Eye Diseases RAMSState Research Institute of Eye Diseases RAMS
A A Kasparov
State Research Institute of Eye Diseases RAMSState Research Institute of Eye Diseases RAMS
A S Pavliuk
The Russian State Medical UniversityThe Russian State Medical University
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