Personalized cell therapy in ophthalmology. I. Method for obtaining and phenotype of autologous product
- Авторлар: Avetisov SE1, Subbot AM2, Antochin AI2, Kasparova EA1, Kasparov AA1, Pavliuk AS2
-
Мекемелер:
- State Research Institute of Eye Diseases RAMS
- The Russian State Medical University
- Шығарылым: Том 6, № 2 (2011)
- Беттер: 38-42
- Бөлім: Articles
- ##submission.dateSubmitted##: 11.01.2023
- ##submission.datePublished##: 15.06.2011
- URL: https://genescells.ru/2313-1829/article/view/121656
- DOI: https://doi.org/10.23868/gc121656
- ID: 121656
Дәйексөз келтіру
Аннотация
autologous leucocytes from peripheral blood for corneal
diseases treatment is developed. Method allows us to obtain
a product with sufficient content of viable nucleated cells (up
to 3106 cell/ml). Cellular composition of the product was
determined. Mononuclear fraction decreased in 1,3 times and
granulocyte portion is increased. Phenotype of lymphocytes
in the cell product is the same as in peripheral blood, with
selective loss of B-lymphocytes. The presence of CD14+/34low
cells, which are considered to be progenitors, is defined in the
product. Using of cell product, obtained by designed technique,
may be promising in clinic, because of it minimal traumatism
for the patient and simplicity.
Авторлар туралы
S Avetisov
State Research Institute of Eye Diseases RAMSState Research Institute of Eye Diseases RAMS
A Subbot
The Russian State Medical UniversityThe Russian State Medical University
A Antochin
The Russian State Medical UniversityThe Russian State Medical University
E Kasparova
State Research Institute of Eye Diseases RAMSState Research Institute of Eye Diseases RAMS
A Kasparov
State Research Institute of Eye Diseases RAMSState Research Institute of Eye Diseases RAMS
A Pavliuk
The Russian State Medical UniversityThe Russian State Medical University
Әдебиет тізімі
- Patel S.A., King C.C., Lim P.K. et al. Personalizing stem cell research and therapy: the arduous road ahead or missed opportunity? Curr. Pharmacog. Person Med. 2010; 8(1): 25-36.
- Romagnani P., Lasagni L., Romagnani S. Peripheral blood as a source of stem cells for regenerative medicine. Expert. Opin. Biol. Ther. 2006; 6(3): 193-202.
- Seta N., Kuwana M. Human circulating monocytes as multipotential progenitors. Keio. J. Med. 2007; 56(2): 41-7.
- Romagnani P., Annunziato F., Liotta F. et al. CD14+CD34low cells with stem cell phenotypic and functional features are the major source of circulating endothelial progenitors. Circ. Res. 2005; 97(4): 314-22.
- Bader A., Macchiarini P. Moving towards in situ tracheal regeneration: the bionic tissue engineered transplantation approach. J. Cell. Mol. Med. 2010; 14(7): 1877-89.
- Parikh C.H., Edelhauser H.F. Ocular surgical pharmacology: corneal endothelial safety and toxicity. Curr. Opin. Ophthalmol. 2003; 14(4): 178-85.
- Alvarado J.A., Katz L.J., Trivedi S. et al. Monocyte modulation of aqueous outflow and recruitment to the trabecular meshwork following selective laser trabeculoplasty. Arch. Ophthalmol. 2010; 128(6): 731-7.
- Бурунова В.В. Проблемы стандартизации при получении клеточных культур мезенхимального происхождения: эксперименталь-ный и теоретический анализ (диссертация). Москва; 2011.
- Sugiyama T., Hoshino K., Saito M. et al. Immunoadjuvant effects of polyadenylic:polyuridylic acids through TLR3 and TLR7. Int. Immunol. 2008; 20(1): 1-9.
- Суббот А.М., Каспарова Е.А., Каралкин П.А. и соавт. // Ство- ловые клетки и регенеративная медицина /под редакцией В.А. Тка- чука. М.: МАКС ПРЕСС, 2011.- С. 162-172.
- Ковальчук Л.В., Павлюк А.С., Синюхин В.Н. и др. Анализ фармакологических средств на модели апоптоза лимфоцитов чело- века in vitro в норме и при иммунопатологии Аллергология и имму- нология 2000; 1: 24-30.
- Kavai T., Akira S. TLR signaling. Cell Death Differ. 2006; 13(5): 816-25.
- Boyum A. Separation of lymphocytes, lymphocyte subgroups and monocytes: a review. Lymphology 1977; 10(2): 71-6.
- Coulson A.S., Chalmers D.G. Separation of viable lymphocytes from human blood. Lancet 1964; 1: 468-9.
- Zhang Z., Schluesener H.J. Mammalian toll-like receptors: from endogenous ligands to tissue regeneration. Cell Mol. Life Sci. 2006; 63(24): 2901-7.
- Jaremko K.M., Chen-Roetling J., Chen L. et al. Accelerated hemolysis and neurotoxicity in neuron-glia-blood clot co-cultures. J. Neurochem. 2010; 114(4): 1063-73.
Қосымша файлдар
![](/img/style/loading.gif)