Treatment of the amyotrophic lateral sclerosis using of genetically modified umbilical cord bloodmononuclear cells in the preclinical studies

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Abstract

Development of the fundamental and clinical «regenerative
medicine» is based on the progress of gene, stem cell and
gene-cell biotechnologies. However, the reliable preclinical
investigations on animal models and more over clinical trials
stay far away from the available nowadays gene and cell
constructions. Neuroscience is one of the fast growing fields
of knowledge in biology and medicine. Pioneer experiments
in neuroscience promises breakthrough in the innovative
methods for treatment of neurodegenerative diseases in
near future. This review addresses strategies for gene-cell
therapy of neurodegenerative diseases by the example of
amyotrophic lateral sclerosis. Precisely gene modification of
mononuclear fraction of umbilical cord blood cells (UCBC)
by dual cassette plasmid vectors is observed. Based on
our own results of transplantation of genetically modified
UCBC overexpressing recombinant neural cell adhesion
molecule L1, vascular endothelial growth factor, fibroblast
growth factor 2, and glial derived neurotrophic factor in
different combinations we provide the experimental data
for usefulness of transplantation of gene modified UCBC
for treating neurodegenerative diseases. In the review we
discuss the efficacy of gene modification of UCBC not only
for secretion of recombinant proteins, but in increasing of
transplanted cells survivability, their migration possibilities
and capability to differentiate in endothelial, microglial and
macroglial cell types.

About the authors

A A Rizvanov,

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

D S Guseva,

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

I I Salafutdinov,

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

F V Bashirov,

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

A P Kiiasov,

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

R R Islamov

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

Kazan (Volga region) Federal University, KazanKazan State Medical University, KazanStem cell bank of the Kazan State Medical University, KazanRepublican Clinical Hospital of the MH RT, Kazan

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