Influence of Hepatic Stellate Cells transduction by adenoviral vector Ad5-optHGF-optFGF-4-RFP on their phenotype in vitro and in vivo



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Abstract

Nowadays gene and cell therapy methods for liver diseases treatment are being actively developed. Genetic modification of cells could be an approach that considerably increases the therapeutic potential of transplanted cells. It is assumed that the genetic manipulation, particularly obtaining and application of cells, that express and overexpress therapeutic factors, could reduce the therapeutic dose of transplanted cells and noticeably enhance therapeutic effects of these cells. However, it remains unclear, how genetic modification influences on cellular phenotype, in this case hepatic stellate cells, and what kind of therapeutic effect will give these cells after transplantation into the organism. The aim of our work was to study the phenotype of hepatic stellate cells after genetic modification by the adenoviral vector Ad5-optHGF-optFGF-4-RFP in vitro and in vivo and after subsequent transplantation into the rats with partial hepatectomy. As a result, it was confirmed that transplantation of hepatic stellate cells, transduced with therapeutic genes, has a positive influence on the process of liver regeneration while the morphology and phenotype of cells remain unchanged. So, we can make a conclusion of safety of this method for use in regenerative medicine

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About the authors

E. I Sharipova

Kazan (Volga region) Federal University; Kazan State Medical University

Email: Elwishdoc@gmail.com

A. A Titova

Kazan (Volga region) Federal University

A. K Shafigullina

Kazan (Volga region) Federal University

A. R Galyavieva

Kazan (Volga region) Federal University; Kazan State Medical University

E. E Garanina

Kazan (Volga region) Federal University

M. O Mavlikeev

Kazan (Volga region) Federal University

G. O Pevnev

Kazan (Volga region) Federal University

G. R Burganova

Kazan (Volga region) Federal University

M. A Titova

Kazan (Volga region) Federal University

A. A Gumerova

Kazan (Volga region) Federal University

A. P Kiyasov

Kazan (Volga region) Federal University

A. A Rizvanov

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