"Stembrid" is a new technology for reprogramming the nucleus of an adult cell without the use of embryos

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About the authors

A. V. Bersenev

Author for correspondence.
Email: redaktor@celltranspl.ru
Russian Federation

References

  1. Jeanisch R., Eggan К., Humpherys D. et al. Nuclear cloning, stem cells, and genomic reprogramming. Cloning Stem Cells 2002; 4: 389-96.
  2. Hwang W.S., Lee B.C., Lee C.K., Kang S.K. Cloned human embryonic stem cells for tissue repair and transplantation. Stem Cell Reviews 2005; 2: 99-110.
  3. Tada M„ Takahama Y., Abe K. et al. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Current Biology 2001; 11:1553-8.
  4. Cowan C.A., Atienza J., Melton D.A., Eggan K. Nuclear reprogramming of spermatic cells after fusion with human embryonic stem cells. Science 2005; 309: 1369-73.
  5. Yu J., Vodyanik M.A., He P. et al. Human embryonic stem cells reprogram myeloid precursors following cell-cell fusion. Stem Cells 2006; 24[1): 168-76
  6. Veomett G., Prescott D.M., Shay J.W., Porter K.R. Reconstruction of mammalian cells from nuclear and cytoplasmic components separated by treatment with cytochalasin B. Proc. Natl. Acad. Sci. USA 1974; 71:1999-2002.
  7. Wallace D.C., Bunn C.L., Eisenstadt J.M. Cytoplasmic transfer of chloramphenicol resistance in human tissue culture cells. J. Cell Biol. 1975; 67: 174-88.
  8. Howell A.N., Sager R. Tumorigenicity and its suppression in cybrids of mouse and Chinese hamster cell lines. Proc. Natl. Acad. Sol. USA 1978; 75: 2358-62.
  9. Abken H., Jungfer H., Albert W.H., Willecke K. Immortalization of human lymphocytes by fusion with cytoplasts of transformed mouse L cells. J. Cell Biol. 1986; 103: 795-805.
  10. Atsumi T., Shirayoshi Y., Takeichi M., Okada T.S. Nullipotent teratocarcinoma cells acquire the pluripotency for differentiation by fusion with somatic cells. Differentiation 1982; 23: 83-6.
  11. Do J.T., Scholer H.R. Nuclei of embryonic stem cells reprogram somatic cells. Stem Cells 2004; 22: 941-9.
  12. Take! S., Yamamoto M„ Cui L. et al. Phenotype-specific cells with proliferative potential are produced by polyethylene glycol-induced fusion of mouse embryonic stem cells with fetal cardiomyocytes. Cell Transplant. 2005; 14[9): 701-8.

Supplementary files

Supplementary Files
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1. JATS XML
2. Fig. 1. "Stembrid" colony - coloration on TRA-2-39 (L-alkaline phosphatase) (green), Oct-4 (red), DAPI (blue) Photo provided by N. Strelchenko

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