The structure, functions and viability of the umbilical cord blood nuclear cells after cryoconservation



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Abstract

New efficient methods for separation and cryopreservation of nucleated cells from umbilical cord blood, which allow you to save a quantitative and qualitative composition of nucleated cells (including hematopoietic stem] after discharge, and demonstrate a high level of preservation of structural and functional properties and viability of CD45+ and CD34+-cells after cryopreservation.

About the authors

L A Babiychuk

V I Grishchenko

P M Zubov

O L Zubova

V V Ryazantsev

L V Babiychuk

O V Kudokotseva

S A Lyubchich

L A Babyichuk

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

V I Grishchenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

P M Zubov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

O L Zubova

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

V V Ryazantsev

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

L V Babyichuk

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

O V Kudokovtseva

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

S A Lyubchich

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

References

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  4. Cornetta К., Laughlin М., Carter S. et al. Umbilical cord blood transplantation in adults: results of the prospective Cord Blood Transplantation (COBLT1. Biol. Blood Marrow Transplant. 2005; 11(21: 149-60.
  5. Владимирская Е.Б., Майорова О.А., Румянцев С.А., Румянцева А.Г. Биологические основы и перспективы терапии стволовыми клетками. 2005. М.: Медпрактика: 391.

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