Some possible molecular mechanisms of VEGF encoding plasmids functioning

Resumo

Gene therapeutic approaches to the restoration of the
ischemic tissue perfusion are considered very promising,
but to this time the molecular mechanisms which allow the
therapeutic gene encoding plasmid to transfect the target
cell and underlie the positive clinical effects remain unknown.
In this review the possible molecular mechanisms of the
angiogenic factor VEGF encoding plasmid penetration into the
cytoplasm and the nucleus of the target cell are discussed,
and also the methods for better transfection and the gene of
interest expression are proposed.