Long noncoding RNA expression pattern in the X inactivation center of rodents



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Abstract

Transcriptome analysis of several cell lines with different differentiation degree has been carried out in three rodent species - voles, rats, and mice. As a result, expression profiles of X-inactivation center genes have been generated. In mice, new data on how expression of key genes of X-inactivation center, Xist and Tsix, changes during ontogenesis have been obtained. New types of transcripts have been revealed. In voles, Xist expression was observed in all 8 cell lines examined. Interestingly, main vole Xist transcript had 7 exons as those of rats and mice. In addition, Xist antisense transcript, Tsix, was expressed in 4 vole cell lines and was presented by both spliced and unspliced variants. Expression between the Enox (Jpx) and Xist genes has been revealed in three vole cell lines. Additional variants of Tsix transcripts were observed when studied expression profiles of several rat cell lines. The variants were not present in mice and voles. Compared to mice and voles, Tsix expression level in rats was several times higher than that of Xist.

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About the authors

E. A Elisaphenko

Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences; Institute of Chemical Biology and Fundamental Medicine, the Siberian Branch of the Russian Academy of Sciences; State Research Institute of Circulation Pathology, Ministry of Healthcare of the Russian Federation

Email: antares@bionet.nsc.ru
Novosibirsk, Russia

A. I Shevchenko

Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences; Institute of Chemical Biology and Fundamental Medicine, the Siberian Branch of the Russian Academy of Sciences; State Research Institute of Circulation Pathology, Ministry of Healthcare of the Russian Federation

Email: antares@bionet.nsc.ru
Novosibirsk, Russia

S. M Zakian

Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences; Institute of Chemical Biology and Fundamental Medicine, the Siberian Branch of the Russian Academy of Sciences; State Research Institute of Circulation Pathology, Ministry of Healthcare of the Russian Federation; National Research University Novosibirsk State University

Email: antares@bionet.nsc.ru
Novosibirsk, Russia

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