The effects of the male sex hormone (testosterone) on the functional activity of T-lymphocytes different degrees of differentiation



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Abstract

The aim of the study was a comprehensive assessment of testosterone effects on the functional activity of T lymphocytes of different differentiation degrees (naive -CD45R + and primed -CD45RO+). Material and methods. CD45RA+ and CD45RO+ T cells obtained from a suspension of mononuclear cells from healthy donors (by immunomagnetic separation) were used as a study material (n = 48). The activation model which reflects the interaction of T lymphocytes of different differentiation degrees with antigen-presenting cells (CD2/CD3/CD28-complex activated T cells) was used to assess dose-dependent effects of testosterone on functional activity of T memory cells of different differentiation degrees. Viability assessment and identification of surface molecules CD25, CD71, CD95 on T cells of different differentiation degrees were performed by flow cytometry; the concentration of IL-2 in supernatant cell cultures was performed by enzyme immunoassay; assessment of the relative mRNA expression level of the telomerase catalytic subunit hTERT gene was performed by polymerase chain reaction. Statistical analysis was made using IBM SPSS Statistics 20 (Statistical Package for the Social Sciences). Results. The proapoptotic effect of testosterone on CD2/ CD3 / CD28-activated primed (CD45RO+) T cells has been established that may be due to nongenomic effects of the male sex hormone. Testosterone-induced changes of the system parameters IL-2/IL-2Ra induced by activated T-cells different degrees of differentiation is unidirectional, have different rates and depend on concentration of the hormone.. Suppressive effects of testosterone largely affect naive (CD45RA+) T cells. Dose-dependent effects of testosterone on the telomerase catalytic subunit (hTERT) gene expression in the background of antigen-independent activation are multidirectional and determined by the degree of T cells differentiation.

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About the authors

V. V Shupletsova

I. Kant Baltic Federal University

Kaliningrad, Russia

O. G Khaziakhmatova

I. Kant Baltic Federal University

Kaliningrad, Russia

A. A Gutsol

I. Kant Baltic Federal University

Kaliningrad, Russia

N. A Sokhonevich

I. Kant Baltic Federal University

Kaliningrad, Russia

K. A Yurova

I. Kant Baltic Federal University

Kaliningrad, Russia

L. S Litvinova

I. Kant Baltic Federal University

Kaliningrad, Russia

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