Comparative characteristic of immunomodulatory properties of human multipotent mesenchymal stromal cells and fibroblasts

Cover Page


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Immunomodulated properties of multipotent mesenchymal stromal cells (MMSC) and fibroblasts mediated both by soluble factors and cell-cell contacts, as well as their reparative potential, are important for use these cells in «regenerative medicine». The morphological and phenotypic characteristics and estimation of the bone marrow- and adipose-derived MMSC (bmMMSC and adMMSC), dermal and postnatal fibroblasts (Foreskin line) influence on mitogen-induced T-lymphocytes proliferation following co-cultivation with donor allogenic peripheral blood mononuclear cells with further calculation of index of MMSC suppression and supernatant's tumor necrosis factor-а concentration. The mesenchymal origin cell cultures have morphophenotypic similarity. The immunosuppressive effect of bone marrow-derived MMSC and Foreskin line's fibroblasts was similar and significantly exceed the ability of adMMSC and dermal fibroblasts to inhibit lymphocyte proliferation. MMSC and fibroblasts constitutively produced low level of tumor necrosis factor-а. However the bone marrow-derived MMSC and Foreskin line's fibroblasts inhibited the tumor necrosis factor-а production by activated peripheral blood mononuclear cells but did not influence on cytokine production by unstimulated cells, whereas the adMMSC significantly increased the cytokine concentrations in all cultures. Dermal fibroblasts did not significantly affect on immune cells secretory activity. The immunomodulatory properties of human adMMSC should be accounted under their use in regenerative medicine.

Full Text

Restricted Access

About the authors

M. M Zafranskaya

Belarusian Medical Academy of Post-Graduate Education, Minsk, Belarus

V. G Bogdan

Belarusian State Medical University, Minsk, Belarus

Yu. E Demidchik

Belarusian Medical Academy of Post-Graduate Education, Minsk, Belarus

Yu. M Gain

Belarusian Medical Academy of Post-Graduate Education, Minsk, Belarus

S. S Bagatka

Belarusian Medical Academy of Post-Graduate Education, Minsk, Belarus

S. E Shelkovich

Belarusian Medical Academy of Post-Graduate Education, Minsk, Belarus

G. I Ivanchik

Belarusian Medical Academy of Post-Graduate Education, Minsk, Belarus

References

  1. Егиев В.Н., Сологуб В.К., Чижов Д.В. и др. Сравнительная оценка степени фиксации фибробластов на синтетических эндопротезах, используемых для пластики дефектов передней брюшной стенки. Герниология 2006; 2: 37-41.
  2. Карпюк В.Б., Перова М.Д., Козлов В.А. и др. Экспериментальная модель реконструкции кости путем остеогенной трансформации аутотрансплантированных свежевыделенных стромальных клеток жировой ткани. Анналы пластической, реконструктивной и эстетической хирургии 2007; 4: 14-18.
  3. Skala С.Е., Petry I.B., Gebhard S. et al. Isolation of fibroblasts for coating of meshes for reconstructive surgery: differences between mesh types. Regen. Med. 2009; 4(2): 197-204.
  4. Мальцева Н.В., Панченко В.А., Прокопьева Е.Г. и др. Биоматрица на основе полипропиленовой сетки и эмбриональных фи-бробластов. Клеточные технологии в биологии и медицине 2008; 3: 128-31.
  5. Haniffa M., Wang X.N, Holtick U. at al. Adult human fibroblasts are potent immunoregulatory cells and functionally equivalent to mesenchymal stem cells. J. Immunol. 2007; 179(3): 1595-604.
  6. Кругляков П.В., Лохматова Е.А., Климович В.Б. и др. Мезенхимальные стволовые клетки и иммунопатологияеские состояния организма. Клеточная трансплантология и тканевая инженерия 2006; ШС5): 36-41.
  7. Aggarwal S., Pittenger M.F. Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood 2005; 105(4): 1815-22.
  8. Wada N., Bartold P.M., Gronthos S. Human foreskin fibroblasts exert immunomodulatory properties by a different mechanism to bone marrow stromal/stem cells. Stem Cells Dev. 2011; 20(4): 645-59.
  9. Jones S., Horwood N., Cope A. et al. The antiproliferative effect to mesenchymal stem cells is a fundamental property shared by all stromal cells. J. Immunol. 2007; 179(5): 2824-31.
  10. Ishii M., Koike C., Igarashi A. et al. Molecular marker distinguish bone marrow mesenchymal stem cells from fibroblasts. Biochem. Biophys. Res. Commun. 2005; 332(1): 297-303.
  11. Yanez R., Lamana M.L., Garcia-Castro J. et al. Adipose tissue-derived mesenchymal stem cells have in vivo immunosuppressive properties applicable for the control of the draft-versus-host disease. Stem Oell 2006; 24(11): 2582-91.
  12. Салина Т.Ю., Морозова Т.И. Особенности продукции фактора некроза опухоли а при туберкулезе легких и внелегочных локализаций. Цитокины и воспаление 2010; 1: 36-42.
  13. Kim Y.S., Park H.J., Hong M.H. et al. TNF-alpha enhances engraftment of mesenchymal stem cells into infarcted myocardium. Front. Biosci. 2009; 14(1): 2845-56.
  14. Abraham D.J., Shiwen X., Black C.M. et al. Tumor necrosis factor alpha suppresses the induction of connective tissue growth factor by transforming growth factor-beta in normal and scleroderma fibroblasts. J. Biol. Chem. 2000; 275(20): 15220-5.
  15. Кевра М.К. Фактор некроза опухолей: изучение роли в организме. Мед. новости. 1995; 8: 13-22.
  16. Haniffa M., Collin M., Buckley C. et al. Mesenchymal stem cells: the fibroblasts' new clothes? Haematologica 2009; 94(2): 258-63.
  17. Bartholomeew A., Sturgeon C., Siatskas M. et al. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo. Exp. Hematol. 2002; 30(1): 42-8.
  18. Scolding N, Marks D, Rice C. Autologous mesenchymal bone marrow stem cells: practical considerations. J. Neuro. Sci. 2008; 265(1-2): 111-5.
  19. Lanza C., Morando S., Voci A. et al. Neuroprotective mesenchymal stem cells are endowed with a potent antioxidant effect in vivo. J. Neurochem. 2009; 110(5): 1674-84.
  20. Krampera M., Cosmi L. Angeli R. et al. Role of interferon-y in the immunomodulatory activity of human bone marrow mesenchymal stem cells. Stem Cells 2006; 24(2): 386-98.
  21. Реброва О.Ю. Статистический анализ медицинских данных. Применение пакета прикладных программ STATISTICA. М.: МедиаСфера; 2002.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2013 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: 

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies