Extraocular muscles of mdx-mice as a target for cellular therapy: the histological characteristic with use computer morphometrical analysis
- Authors: Avetisov S.E.1, Pavliuk A.S.1,2, Stenina M.A.2, Fedorov A.A.1, Krivov L.P.2, Nikolaenko D.S.1, Baranov P.Y.1, Subbot A.M.1, Tuhvatulin A.I.1
-
Affiliations:
- Research Institute of Eye Diseases RAMS
- Staat Medical University of Russia
- Issue: Vol 3, No 3 (2008)
- Pages: 47-50
- Section: Original Study Articles
- URL: https://genescells.ru/2313-1829/article/view/166052
- ID: 166052
Cite item
Open Access
Access granted
Abstract
Using computer-assisted morphometrycal analysis we show degeneration and regeneration processes in mdx-mouse extraocular muscles. We¢ve also selected statistical criteria for assessment of degeneration rate. Тhus we suggest to use mdx-mouse extraocular muscles as experimental bioilogical model of myodegenerative processes in human extraocular muscles in search, development and effectiveness assessment of different ways of treatment, among them cell-based and gene therapy.
Keywords
Full Text
About the authors
S. E. Avetisov
Research Institute of Eye Diseases RAMS
Email: pavliukster@gmail.com
Russian Federation, Moscow
A. S. Pavliuk
Research Institute of Eye Diseases RAMS; Staat Medical University of Russia
Author for correspondence.
Email: pavliukster@gmail.com
Russian Federation, Moscow; Moscow
M. A. Stenina
Staat Medical University of Russia
Email: pavliukster@gmail.com
Russian Federation, Moscow
A. A. Fedorov
Research Institute of Eye Diseases RAMS
Email: pavliukster@gmail.com
Russian Federation, Moscow
L. P. Krivov
Staat Medical University of Russia
Email: pavliukster@gmail.com
Russian Federation, Moscow
D. S. Nikolaenko
Research Institute of Eye Diseases RAMS
Email: pavliukster@gmail.com
Russian Federation, Moscow
P. Y. Baranov
Research Institute of Eye Diseases RAMS
Email: pavliukster@gmail.com
Russian Federation, Moscow
A. M. Subbot
Research Institute of Eye Diseases RAMS
Email: pavliukster@gmail.com
Russian Federation, Moscow
A. I. Tuhvatulin
Research Institute of Eye Diseases RAMS
Email: pavliukster@gmail.com
Russian Federation, Moscow
References
- Каспаров А.А. Офтальмогерпес. М.: Медицина; 1994.
- Ярыгин В.Н., Сухих Г.Т., Ситников В.Ф. и др. Ксенотрансплантация эмбриональных предшественников миогенеза человека для коррекции дистрофинопатии у мышей с наследственной миодистрофией. Бюллетень экспериментальной биологии и медицины 2003; 136 (7): 100-2.
- Ярыгин В.Н., Стенина М.А., Булякова Н.В. и др. Восстановление икроножной мышцы мышей mdх разного возраста после травмы и при имплантации ксеногенной мышечной ткани. Бюллетень экспериментальной биологии и медицины 2006; 142 (В): 216-8.
- Сукач А.М. Перспективы использования генной и клеточной терапий для лечения мышечных дистрофий. Клеточная трансплантология и тканевая инженерия 2006; 2 (4): 44-50.
- Ferrari G., Cusella-De Аngelis G., Coletta M. et al. Muscle regeneration by bone marrow-derived myogenic progenitors. Science 1998; 279: 1528-30.
- Вulfield G., Siller W.G. et al. Х chromosome-linked muscular dystrophy (mdх) in the mouse. PNАS USА 1984; 81: 1189-92.
- Кhurana T.S., Prendergast R.А., Аlameddine Н.S. et al. Аbsence of Eхtraocular Muscle Pathology in Duchenne’s Muscular Dystrophy: Role for Calcium Нomeostasis in Eхtraocular Muscle Sparing. J. Eхp. Med. 1995; 182: 467-75.
- Porter J.D., Rafael J.А., Ragusa R.J. et al. The sparing of eхtraocular muscle in dystrophinopathy is lost in mice lacking utrophin and dystrophin. J. Cell Science 1998; 111: 1801-11.
- Porter J.D., Merriam А.P., Sangeeta К. et al. Constitutive properties, not molecular adaptations, mediate eхtraocular muscle sparing in dystrophic mdх mice. FАSEВ J. 2003; 17: 893-5.
- Вudak M.T., Вogdanovich S., Wiesen-Martin Н.J. et al. Layerspecific differences of gene eхpression in eхtraocular muscles identified by laser-capture microscopy. Physiol. Genomics 2004; 20: 55-65.
- Chambers R.L., McDennott J.C. Molecular basis of skeletal muscle regeneration. Can. J. Аppl. Physiol. 1996; 21 (3): 155-84.
Supplementary files
