Extracellular ATP molecules effects the functional properties of granulocyte plasma membrane



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Abstract

Extracellular ATP is an auto- and paracrine regulator in the mechanisms of intercellular signaling. It is the trigger starting the purinergic signaling cascade also. The aim of this work to study the effect of extracellular ATP on the functional properties (rigidity, surface potential, adhesive properties and osmoregulatory capabilities of the membrane) of the plasma membrane and the migration activity of granulocytes in experiments in vitro. In the experiment, the granulocyte subpopulation separated from the venous blood of healthy people was used. The experiment samples were incubated with adenosine-5-triphosphate disodium salt trihydrate in concentration 10 мМ that matches of ATP concentration released from blood cells during deformation stress in the microvasculature vessels. The stiffness and surface potential of a cell, the adhesion force between erythrocyte and granulocyte was measured by using the method of atomic force microscopy, tests with hypoosmotic load were performed and the migration activity of granulocytes was studied. As a result of the experiment, a decrease in stiffness and surface potential was found by 53.2% and 32.5 % (р<0.05) respectively, an increase the adhesion force between erythrocyte and granulocyte by 43.3 (p<0.05) and migration activity of the cell by 74.8% (p<0.05) compared with the control. In the hypoosmotic tests, the use of membrane reserve by granulocytes was decreased during whole time incubation compared with control under the influence of extracellular ATP. Obtained data point out to a key role of extracellular ATP on inflammation development in the vascular wall and they are able be taken into account during elaboration pharmacological regulatory targets aimed at the restoration of endothelium dysfunction.

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M. Yu Skorkina

Belgorod State National Research University

Email: skorkina@bsu.edu.ru

T. S Shevchenko

Belgorod State National Research University

V. V Fetter

Belgorod State National Research University

O. V Cherkashina

Sv. loasaf Belgorod Region Hospital

M. Yu Palchikov

Belgorod State National Research University

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