The possibility of using human hormones for maturation of swine oocytes in vitro

Gonadotropins have been used for the last 50 years in assisted reproductive technologies. Often, due to hyperstimulation, there is a worsening effect on both eggs and patients. Therefore, it is possible to use the swine oocyte maturation system in vitro as a culture model for testing hormones used in humans. The purpose of our research was to study the possibility of using human chorionic gonadotropin for maturation of swine oocytes in vitro. The criterion of successful maturation was the presence of the first polar body (MII stage), as well as the ability of matured oocytes to reach the blastocyst stage after artificial (parthenogenetic) activation. It is shown that in the medium with Ovogest(human chorionic gonadotropin derived from urine) there is a tendency to improve the maturation of the nucleus compared to the variant with Pregnyl(human chorionic gonadotropinderived from urineandused for "human” in vitro fertilization) - 59,5% vs 45,8%. After artificial activation, the oocytes reached the stage MII, the cleavage is approximately the same in both cases (89,4% vs 81,2%). But, the percentage of blastocysts obtained, both from the total number of activated eggs, and from the number of cleaving after activation, was greater in variant with Pregnyl(42,4% vs 23,4% without significant difference and 51,2% vs 26,2% with significant difference р<0,05, respectively). Our results show that human chorionic gonadotropin, used for "human” in vitro fertilization, can be used for maturation of swine oocytes in vitro, which is confirmed by the successful use of eggs in experiments on parthenogenetic activation of embryos. It is also possible to use the swine oocyte maturation system in vitro as a culture model for testing hormones used in human in vitro fertilization programs.

oocytes, in vitro maturation, swine, human chorionic gonadotropin