Phenotype and functional properties of adipose derived MMSC after interaction with cord blood mononuclear cells

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Abstract

It is well known, that ex vivo expansion of hematopoietic stem cells is more effective when the culture conditions mimic their in vivo microenvironment. Multipotent mesenchymal stromal cells (MMSC) are widely used in such systems due to their ability to support hematopoiesis. In addition, the result of MMSC-hematopoietic cells' interaction can be affected by O 2 concentration, which is shown to modify the number of MMSC characteristics. The feedback effects of hematopoietic cells on MMSC during their interactions are less investigated. We studied the effects of cord blood mononuclear cells (MNC] and MMSC interaction on the later cells morphofunctional characteristics during 72 h coculture at 20% or 5% O 2. The dynamics of adhesion was estimated with CCD-camera equipped microscopy, the effects of the interaction with MNC on MMSC viability and CD54, CD44, CD90 expression were detected by flow cytometry. After interaction with MNC, MMSC displayed increased CD54 and in some cases - CD44 expression. The share of CD90+-MMSCs and their viability remained high during coculture with MNC at both O 2 levels. The adhesion of MNC was somewhat faster in 20% O 2. Thus, regardless of O 2 concentration coculture of allogenic MMSCs and MNC inc 2reased the expression of MMSC adhesion molecules involved in formation of specialized cell-to-cell contacts that provided effective adhesion of MNC. MMSC did not provoke allogenic MNC cytotoxic activity that is of particular importance in the case of potential therapeutic application of MMSC.

About the authors

P. I Bobyleva

Institute of Biomedical Problems of the RAS, Moscow

I. V Andrianova

Institute of Biomedical Problems of the RAS, Moscow

E. V Maslova

Institute of Biomedical Problems of the RAS, Moscow

E. R Andreeva

Institute of Biomedical Problems of the RAS, Moscow

BSh. Sh Gogiya

Institute of Biomedical Problems of the RAS, Moscow

L. B Buravkova

Institute of Biomedical Problems of the RAS, Moscow

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