Gene transfer to mice organs using non-viral systems for targeted delivery with differenthydrophobicity and with lactose addressing group
- Authors: Bogdanenko EV1, Ibragimova MY.2, Zinnatullina ET2, Shakirova RI2, Khramova AY2, Zhdanov RI1,2,3
-
Affiliations:
- SRI of General Pathology and Pathophysiology of RAMS, Moscow
- Kazan (Volga region) federal university, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 29-33
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121570
- DOI: https://doi.org/10.23868/gc121570
- ID: 121570
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Abstract
Biodistribution of lipoplexes formed of cholenim substances
I-III, containing one, two or three cholesterol moieties, and
eukaryotic 14С-DNA and(or) reporter gene into mice organs
using a variety of administration routes (intraperitoneally,
i.p.; portal vein or left renal artery) is studied in this paper. It
is shown that biodistribution doesnt depend on lipoplex lipid
composition under i.p. administration, and depends on lipid
nature under vein and artery administration. Effective in vivo
transfection and reporter gene expression are demonstrated
under portal vein administration of lipoplex formed of
dicholenim II and lactosylated lipid IV (1 to 1 mass ratio). In the
case, the β-Gal gene expression (above 0,3 mcg / g of tissue)
is demonstrated in lungs, liver and spleen histochemically
and spectrophotometrically. Introduction of cholesterol
moieties into oligoethylenimine structure results in optimal
hydrophilicity/hydrophobicity ratio, their stabilization, and
optimal value of critical constant of micelle formation. There
are certain outlooks due to usage of the lipoplexes described
for targeted gene delivery.
I-III, containing one, two or three cholesterol moieties, and
eukaryotic 14С-DNA and(or) reporter gene into mice organs
using a variety of administration routes (intraperitoneally,
i.p.; portal vein or left renal artery) is studied in this paper. It
is shown that biodistribution doesnt depend on lipoplex lipid
composition under i.p. administration, and depends on lipid
nature under vein and artery administration. Effective in vivo
transfection and reporter gene expression are demonstrated
under portal vein administration of lipoplex formed of
dicholenim II and lactosylated lipid IV (1 to 1 mass ratio). In the
case, the β-Gal gene expression (above 0,3 mcg / g of tissue)
is demonstrated in lungs, liver and spleen histochemically
and spectrophotometrically. Introduction of cholesterol
moieties into oligoethylenimine structure results in optimal
hydrophilicity/hydrophobicity ratio, their stabilization, and
optimal value of critical constant of micelle formation. There
are certain outlooks due to usage of the lipoplexes described
for targeted gene delivery.
About the authors
E V Bogdanenko
SRI of General Pathology and Pathophysiology of RAMS, MoscowSRI of General Pathology and Pathophysiology of RAMS, Moscow
M Ya Ibragimova
Kazan (Volga region) federal university, KazanKazan (Volga region) federal university, Kazan
E T Zinnatullina
Kazan (Volga region) federal university, KazanKazan (Volga region) federal university, Kazan
R I Shakirova
Kazan (Volga region) federal university, KazanKazan (Volga region) federal university, Kazan
A Y Khramova
Kazan (Volga region) federal university, KazanKazan (Volga region) federal university, Kazan
R I Zhdanov
SRI of General Pathology and Pathophysiology of RAMS, Moscow;Kazan (Volga region) federal university, Kazan;SRI of General Pathology and Pathophysiology of RAMS, Moscow;Kazan (Volga region) federal university, Kazan;
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