Using organotypic cultures of the retina as a model for studying migratory activity of transplanted cells



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Abstract

A lot of experimental data have been accumulated and numerous clinical trials have been conducted in the field of stem cell biology in recent years. But still there are many open questions about the survival of transplanted cells, interaction between transplanted cells and recipient microenvironment. That's why it is necessary to develop experimental models of damaged retina which allow studying the behavior of individual cells after transplantation. We used organotypic explantation culture of newborn rat neuroretina as a model to study unfluence of transplanted cells and trophic factors on neuraretina. The advantages of this model are: visualization of transplanted cells, simple analysis of their morphology, survival capacity in neuroretina, interactions with recipient tissue and migration ability.
We used newborn rat neuroretina explantation culture (DMEM/F12 with 20ng/mlFGFandEGF, 7% FCS and antibiotics). After 10 days of cultivation retina pigment epithelium cells, MMSC and NSPC from the subventricular brain zone of C57BL/ B-TgCACTB-EGFP)/Osb/J GFP+ mice were transplanted into the cultured neuroretina/ explants. The immunohistochemical staining for [i-lll-tubulin and GFAP was carried out.
In our study significant differece in the survival capacity and migration ability of different cell types has been demonstrated, and morphological changes of transplanted cells were have been analyzed.

About the authors

M L Semen

S A Sergeev

I N Saburina

N V Kosheleva

M L Semenova

Lomonosov Moscow State University, Moscow

Lomonosov Moscow State University, Moscow

S A Sergeev

Lomonosov Moscow State University, Moscow

Lomonosov Moscow State University, Moscow

I N Saburina

Institute of General Pathology and Pathophysiology, Moscow

Institute of General Pathology and Pathophysiology, Moscow

N V Koscheleva

Lomonosov Moscow State University, MoscowInstitute of General Pathology and Pathophysiology, Moscow

Lomonosov Moscow State University, MoscowInstitute of General Pathology and Pathophysiology, Moscow

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