Vliyanie ponizhennogo soderzhaniya kisloroda na differentsirovku mul'tipotentnykh mezenkhimal'nykh stromal'nykh kletok kostnogo mozga cheloveka in vitro



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Abstract

Исследовали дифференцировочный потенциал мультипотентных мезенхимальных стромальных клеток [мезенхимальных стволовых клеток, МСК) костного мозга человека и экспрессию транскрипционного фактора, индуцируемого при гипоксии (Hypoxia Inducible Factor - HIF-1 cd, при культивировании в условиях пониженного содержания кислорода (1 и 5% Су. При индукции МСК в нормоксических (20% Су условиях и при пониженном содержании кислорода установлена, проявляющаяся в различной степени, способность МСК к дифференцировке в остеогенном, адипогенном, эндотелиальном направлениях. Методом проточной цитометрии выявлено, что уровень HIF-1 ее в цитоплазме монослойных культур МСК, инкубированных при 1 и 5% содержании кислорода в течение 24 ч и 7 сут., снижается по сравнению с нормоксическим контролем (20% Op).

References

  1. Caplan A.I. Mesenchymal stem cells. J. Orthop Res. 1991; 9 [53: Б41-50.
  2. PittengerM. F., Mackay A.M., Beck S.С etal. Mutilineage potential of adult human mesenchymal stem cells. Science 1999; 284: 143-7.
  3. Semenza G.L., Wang G.L. A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gene enhancer at a site required for transcriptional activation. Mol. Cell Biol. 1992; 12 [12]: 5447-54.
  4. Carmeliet P., Dor Y., Herbert J.M. et al. Role of HIF-1 alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis. Nature. 1998; 394: 485-490.
  5. Semenza G.L. Hypoxia, clonal selection, and the role of HIF-1 in tumor progression. Crit. Rev. Biochem. Mol. Biol. 2000; 35: 71-103.
  6. Б. Greijer A.E., van der Wall E. The role of hypoxia inducible factor 1 [HIF-1] in hypoxia induced apoptosis. J. Clin. Pathol. 2004; 57: 1009-14.
  7. Manalo D.J., Rovan A., Lavoie T. et al. Transcriptional regulation of vascular endothelial cell responses to hypoxia by HIF-1. Blood 2005; 105: 659-69.
  8. B. Wang G.L, Jiang B.H, Semenza G.L. Effect of protein kinase and phosphatase inhibitors on expression of hypoxia-inducible factor 1. Biochem. Biophys. Res. Commun. 1995; 216: 669-75
  9. Maxwell P.H., Wiesener M.S., Chang G.W. et al. The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis. Nature 1999; 399: 271-5.
  10. Yun Z., Maecker H.L., Johnson R.S., Giaccia A.J. Inhibition of PPAR gamma 2 gene expression by the HIF-1-regulated gene DEC1/Stra13: a mechanism for regulation of adipogenesis by hypoxia. Dev. Cell. 2002; 2: 331-41.
  11. Расагу E., LegrosH., ValableS. et al. Synergistic effects of CoCI2 and ROCK inhibition on mesenchymal stem cell differentiation into neuronlike cells. J. Cell Sci. 2006; 119: 2667-78.
  12. Kieda C, Greferath R., Da Silva C.C. et al. Suppression of hypoxia-induced HIF-1 ot and of angiogenesis in endothelial cells by myo-inositol trispyrophosphate-treated erythrocytes. Proc. Natl. Acad. Sci. U. S. A. 2006; 103(42): 15576-81.
  13. Provot S., Zinyk D., GunesY. et al. HIF-16 regulates differentiation of limb bud mesenchyme and joint development. J. Cell Biol. 2007; 177: 451-64.
  14. RamHrez-Bergeron D.L., Simon M. С Hypoxia-inducible factor and the development of stem cells of the cardiovascular system. Stem Cells 2001; 19t4): 279-86.
  15. Pugh C.W., Ratcliffe P.J. Regulation of angiogenesis by hypoxia: role of the HIF system. Nat. Med. 2003; 9: 677-84.
  16. Kelly B.D., Hackett S.F., Hirota K. et al. Cell type-specific regulation of angiogenic growth factor gene expression and induction of angiogenesis in nonischemic tissue by a constitutively active form of hypoxia-inducible factor 1. Circ. Res. 2003; 93(11): 1074-81.
  17. Salim A., Nacamuli R.P., Morgan E.F. et al. Transient changes in oxygen tension inhibit osteogenic differentiation and Runx2 expression in osteoblasts. J. Biol. Chem. 2004; 279: 40007-16.
  18. Буравкова Л.Б., Анохина Е.Б. Мезенхимальные стромальные клетки-предшественники: общая характеристика и особенности функционального состояния при пониженном содержании кислорода. Российский физиологический журнал им. И.М. Сеченова 2008; 94 [71: 737-57.
  19. Fink Т., Abildtrup L., Fogd К. et al. Induction of adipocyte-like phenotype in human mesenchymal stem cells by hypoxia. Stem Cells 2004; 22: 1346-55.
  20. Hermann A., Gastl R., Liebau S. et al. Efficient generation of neural stem cell-like from adult human bone marrow stromal cells. J. Cell Sci. 2004; 117: 4411-22.
  21. Wislet-Gendebien S., Hans G., Leprince P. et al. Plasticity of cultured mesenchymal stem cells: switch from nestin-positive to excitable neuronlike phenotype. Stem cells. 2005; 23 (3): 392-402.
  22. Wang P.P., Wang J.H., Yan Z.P. et al. Expression of hepatocyte-like phenotypes in bone marrow stromal cells after HGF induction. Biochem. Biophys. Res. Commun. 2004; 320: 712-16.
  23. Prockop D.J., Gregory C.A., Spees L. One strategy for cell and gene therapy: harnessing the power of adult stem cells to repair tissues. Proc. Natl. Acad. Sci. USA 2003; 100: 11917-23.
  24. Majumdar M.K., Thiede M.A., Mosca J.D. et al. Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells. J. Cell. Physiol. 1998; 176: 57-66.
  25. Maxwell P.H., Dachs G.U., Gleadle J.M. et al. Hypoxia-inducible factor-1 modulates gene expression in solid tumors and influences both angiogenesis and tumor growth. Proc. Natl. Acad. Sci. USA 1997; 94: 8104-09.
  26. Hollander A.P., Corke K.P., Freemont A.J., Lewis C.E. Expression of hypoxia-inducible factor 16 by macrophages in the rheumatoid synovium. Arthritis Rheum. 2001; 44 [71: 1540-44.
  27. Hitchon C, Wong K., Ma G. et al. Hypoxia-induced production of stromal cell-derived factor 1 (CXCL12) and vascular endothelial growth factor by synovial fibroblasts. Arthritis Rheum. 2002; 46 [10): 2587-97.

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