Vliyanie ponizhennogo soderzhaniya kisloroda na differentsirovku mul'tipotentnykh mezenkhimal'nykh stromal'nykh kletok kostnogo mozga cheloveka in vitro



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Resumo

Исследовали дифференцировочный потенциал мультипотентных мезенхимальных стромальных клеток [мезенхимальных стволовых клеток, МСК) костного мозга человека и экспрессию транскрипционного фактора, индуцируемого при гипоксии (Hypoxia Inducible Factor - HIF-1 cd, при культивировании в условиях пониженного содержания кислорода (1 и 5% Су. При индукции МСК в нормоксических (20% Су условиях и при пониженном содержании кислорода установлена, проявляющаяся в различной степени, способность МСК к дифференцировке в остеогенном, адипогенном, эндотелиальном направлениях. Методом проточной цитометрии выявлено, что уровень HIF-1 ее в цитоплазме монослойных культур МСК, инкубированных при 1 и 5% содержании кислорода в течение 24 ч и 7 сут., снижается по сравнению с нормоксическим контролем (20% Op).

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