Angiogenic properties of myocardial c-kit+ cells

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Abstract

Now, great interest is connected with application of the cells received directly from a myocardium as the tool for stimulation of angiogenesis and reparative processes in heart. The aim of the present research is studying of angiogenic properties in vitro and in vivo of c-kit+-cells, obtained from hearts of small rodents. The work was performed on male C57BL/6 mice and male Wistar rats. The study used methods of cytofluorimetry, immuno-fluorescent staining, models of myocardial infarction and subcutaneous angiogenesis in Matrigel™. It was shown, that after acute myocardial infarction c-kit+ cells activated, part of which entered into endothelial differentiation and integrated into de novo formed vessels. C-kit+-expressed the markers of vascular progenitor cells, exhibited endothelial-like behavior in vitro, and after subcutaneous transplantation increased vascularization of Matrigel™. This is achieved both due to the paracrine effects, which stimulate the growth of the recipient's mouse vessels into the Matrigel implant, and partially due to the differentiation of the transplanted cells into the vascular endothelium cells, which was confirmed by vital dye staining. Thus, c-kit+-cells derived from the myocardium can be considered as a promising type of resident cells exhibiting the properties of progenitor cells of the heart vessels for developing approaches to stimulate vascularization of the ischemic myocardium.

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About the authors

K. V Dergilev

Institute of Experimental Cardiology, National Medical Research Center of Cardiology

Email: doctorkote@gmail.com

Z. I Tsokolaeva

Institute of Experimental Cardiology, National Medical Research Center of Cardiology

I. B Beloglazova

Institute of Experimental Cardiology, National Medical Research Center of Cardiology

E. I Ratner

Institute of Experimental Cardiology, National Medical Research Center of Cardiology

Yu. D Molokotina

Institute of Experimental Cardiology, National Medical Research Center of Cardiology

E. V Parfenova

Institute of Experimental Cardiology, National Medical Research Center of Cardiology; M.V. Lomonosov Moscow State University

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