Magnetophoretic properties of human fetal fibroblasts magnetically labeled with citrate stabilized superparamagnetic iron oxide nanoparticles


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Abstract

Cell transplantology is a perspective and actively developing field of regenerative medicine, but its effectiveness often remains relatively low for some indications. Appliance of magnetic nanoparticles and magnetic fields can increase efficacy of cell transplantation. Superparamagnetic iron oxide nanoparticles (SPION) is a most prospective type of magnetic nanoparticles for magnetically controlled targeting of cells in vivo. In the investigation human fetal fibroblasts were labeled with citrate stabilized SPION (SPION-Cit) that refer to anionic magnetic nanoparticles. 100, 200, 300 and 500 дд Fe/ml doses of magnetic nanoparticles and incubation times of 1, 2 and 3 h were used. The effectiveness of magnetic labeling of cells was evaluated by magnetophoresis in a special chamber using disk NdFeB magnet with diameter of 6 mm and thickness of 3 mm and field induction of 0,255 T: magnetophoretic velocity of magnetized cells in magnetic field gradient was assessed and their magnetic susceptibility was calculated. Viability of magnetically labeled cells was evaluated by trypan blue staining and MTT-test. The value of magnetic susceptibility of magnetically labeled fetal fibroblasts was dose and incubation time depended. A capture distance of labeled cell with the magnet was in a range of 3-4 mm. Magnetophoretic movement of control fibroblasts was not observed. Viability of labeled cells was not decreased substantially in ranges of nanoparticles concentrations 100-300 дд Fe/ml and incubation times 1-3 h. In the concentration of 500 дд Fe/ml partial cell death and exfoliation of cell layer from culture flask observed (signs of low grade exfoliation also observed in the labeling conditions of 300 дд Fe/ml for 3 h). According to our data, most optimal conditions for magnetic labeling of human fetal fibroblast with SPION-Cit is particles concentration 100 дд Fe/ml for 3 h, which provides the capture distance of labeled cells with the magnet about 4 mm.

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About the authors

V. V Turchyn

V.K. Gusak Institute of Urgent and Recovery Surgery

Donetsk

Yu. A Legenkiy

Donetsk National University

Donetsk

M. V Solopov

Donetsk National University

Donetsk

A. G Popandopulo

V.K. Gusak Institute of Urgent and Recovery Surgery

Donetsk

S. V Bespalova

Donetsk National University

Donetsk

I. Ya E.Ya. Fistal

V.K. Gusak Institute of Urgent and Recovery Surgery

Donetsk

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