Cytotoxic activity of T-2 toxin for a immortalized of cattle fetal lung epithelium cells



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Abstract

T-2 toxin produced by microscopic fungi induces apoptosis in cells of various body tissues. The purpose of this study was to assess T-2 toxin cytotoxic effect on fetal calf lung epithelium cells. Cells were cultured in a nutrient medium containing T-2 toxin at a concentration of 6000, 3000, 2000, 1000, 500, 100, 50 and 25 ng/ml for 20 hours. The cytotoxic effect of T-2 toxin was assessed by morphological parameters, proliferative activity and cell survival. It was found that the changes in morphology, proliferation, mitotic activity of cells, as well as their survival depended on the concentration of T-2 toxin. High doses of the toxin prevented the cells to form a monolayer, suppressed proliferation, led to changing of their shape, fragmentation and the high level of apoptosis. At doses of 50 and 100 ng/ml monolayer poorly occurred. At a concentration of 25 ng/ml, the toxin had no significant effect on the studied parameters. Morphological analysis was confirmed by cytometry, results of evaluation of proliferative and mitotic activity, cytotoxicity.

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About the authors

I. I Idiyatov

Federal center of Toxicological, Radiological and Biological Safety

Kazan, Russia

L. R Valiullin

Federal center of Toxicological, Radiological and Biological Safety

Kazan, Russia

V. V Biryulya

Federal center of Toxicological, Radiological and Biological Safety

Kazan, Russia

N. G Shangaraev

Federal center of Toxicological, Radiological and Biological Safety

Kazan, Russia

I. S Raginov

Kazan State Medical University

Kazan, Russia

M. I Tremasov

Federal center of Toxicological, Radiological and Biological Safety

Kazan, Russia

M. V Lekishvili

N.N. Priorov Central Research Institute of Traumatology and Orthopedics

Moscow, Russia

A. I Nikitin

Federal center of Toxicological, Radiological and Biological Safety

Kazan, Russia

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