The dependence of proliferation rate of human dermal fibroblasts on growth medium composition and fetal bovine serum concentration



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Abstract

Application of dermal fibroblasts for therapy requires the use of large volume of cell material. The automatized cultivation provides stable conditions for GMP-grade cell production. To define the effect of serum concentration on proliferation rate, fibroblasts were cultured in growth media with different serum percentage. The proliferation rate was evaluated by the use of average period of cell population doubling. For choosing the optimal culture medium, different types of media together with their mixtures, produced by Gibco and PanEco, were explored. It was shown that exponential decrease of the period of cell population doubling occurs when the serum concentration rises from zero to 1 2%, and further increment of serum percentage is followed only by minor reduction of this value. The highest proliferation rate of dermal fibroblasts is obtained when they are cultivated in aMEM medium or in the mixtures of aMEM with F-12, Advanced DMEM with F-12 and Advanced DMEM with RPMI-1640 media (Gibco, USA).

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About the authors

F. A Fadeyev

Ural State Medical University;Institute of Medical Cell Technologies

Ekaterinburg, Russia

D. V Lugovets

Institute of Medical Cell Technologies

Ekaterinburg, Russia

M. V Ulitko

Institute of Medical Cell Technologies

Ekaterinburg, Russia

S. L Leontyev

Institute of Medical Cell Technologies

Ekaterinburg, Russia

S. V Sazonov

Ural State Medical University; Institute of Medical Cell Technologies

Ekaterinburg, Russia

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