Histological features of the lipograft with platelet-rich plasma after subcutaneous transplantation in vivo

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Abstract

Autologous adipose tissue transplantation is one of the most common methods of soft tissue volume and shape correction However, the effect of fat grafting is short due to the low survival rate of a fat graft This study was designed to evaluate the influence of the platelet-rich plasma (PRP) on the dynamics of involutive changes of the fat graft in an experimental model with subcutaneous injection in the rabbit ear shell Each animal (n = 9) underwent subcutaneous autologous fat grafting: in the left ear - without PRP, in the right one - mixed with PRP We performed the histological analysis of the materials estimating the number of fat elements, fibrosis processes, and severity of the macrophage-histiocytic reaction (identification of CD163+-cells) in 1, 2, 4, 8, 36 weeks after surgery We found that in the early stages of observation, up to 4 weeks, the signs of fibrosis (the thickness of the connective tissue capsule, interlobular and interadipocyte fibrous septums) were less pronounced in the case of PRP However, further histologic characteristic became similar in the both groups Thus, PRP can have a positive impact on the autologous fat graft survival, but more research is needed to select the optimal PRP processing protocol for autologous fat graft and further analysis of the causes, mechanisms and symptoms of the transplanted fat tissue involution

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About the authors

I. R Dzampaeva

A.I. Evdokimov Moscow State University of Medicine and Dentistry

Email: ilonadzampaeva@yahoo.com

I. V Gaivoronskiy

S.M. Kirov Military Medical Academy

I. V Krainik

National Medical Surgical Center

A. Y Drobyshev

A.I. Evdokimov Moscow State University of Medicine and Dentistry

I. Y Bozo

A.I. Evdokimov Moscow State University of Medicine and Dentistry; Human Stem Cells Institute; A.I. Burnazyan Federal Medical Biophysical Center

A. V Glushko

A.I. Evdokimov Moscow State University of Medicine and Dentistry

R. V Deev

Human Stem Cells Institute

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