Cytokines and growth factors genes expression after subtotal liver resection in rats

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Abstract

Liver regeneration after 70% resection is one of the most studied models of tissue regeneration Small residual liver volume after massive hepatectomy or partial liver transplantation is a major cause of subsequent liver dysfunction known in oncology and transplantology as small-for-size syndrome The aim of this study was to evaluate the influence of 80% liver resection on cytokines and growth gens expression in rat Male Wistar rats were subjected to 80% and sacrificed at different times after surgery. Untreated animals served as controls Serum and liver samples were obtained to investigate liver function, cytokines and growth factors genes expression: il1b, il6, il10, tnfa, tweak, mmp9, fgf2, tgfb, hgf, vegf, sdfa, ang with quantitative RT-PCR. It was revealed two phases increased expression of genes that correspond to the first and second peak hepatocytes proliferation The first phase was characterized by increased expression mmp9, il6, il10, fgf2, tgfb. The second phase was characterized by increased expression of hgf, tnfa, tweak, il1b. Furthermore, we have found increased expression of the transcription factor SOX9 gene, and the gene encoding TWEAK, indicating a possible role for resident progenitor cells in the regenerating liver of rats after subtotal resection In our opinion the two phases of increased gene expression during liver regeneration in rats after subtotal resection are associated with two waves of hepatocyte proliferation, and the presence of growth factors reserves in the liver.

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About the authors

A. V Elchaninov

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology; N.I. Pirogov Russian National Research Medical University

T. Kh Fatkhudinov

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology; N.I. Pirogov Russian National Research Medical University

Email: fatkhudinov@gmail.com

N. Y Usman

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology; N.I. Pirogov Russian National Research Medical University

I. V Arutyunyan

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology; N.I. Pirogov Russian National Research Medical University

A. V Makarov

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology; N.I. Pirogov Russian National Research Medical University

E. Y Kananykhina

Research Center for Obstetrics, Gynecology and Perinatology; Scientific Research Institute of Human Morphology

E. Sh Raimova

N.I. Pirogov Russian National Research Medical University

G. B Bolshakova

Scientific Research Institute of Human Morphology

G. T Sukhikh

Research Center for Obstetrics, Gynecology and Perinatology

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