Lymphocyte apoptosis in neonatal sepsis due to klebsiella


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

The apoptosis of lymphocytes was examined in 10 infants with late neonatal sepsis induced by Klebsiella pneumoniae Most of the infants (70%) were preterm. Control group consisted of 7 healthy newborns. Apoptosis analysis was examined by counting the numbers of hypodiploid cells by using a propidium iodide (DNA staining procedure and flow cytometry analysis. We observed an elevated numbers of apoptotic cells in all cases of neonatal sepsis Median of lymphocyte apoptosis was 18 1% on day 5 of cell culture, whereas in control patients it was up to 7,8%. Absolute lymphopenia was observed in 30% of cases with neonatal sepsis. The activity of lymphocyte apoptosis was not associated with quantitative baseline of C-reactive protein (Spearman R = 0,48, p = 0,18). Acute phase of neonatal sepsis induced by Klebsiella pneumonia is associated with an increased apoptosis of peripheral blood lymphocytes

Full Text

Restricted Access

About the authors

Kh. S Khaertynov

Kazan State Medical University

Email: khalit65@rambler.ru

S. V Boichuk

Kazan State Medical University

V. A Anokhin

Kazan State Medical University

B. R Ramazanov

Kazan State Medical University

A. A Rizvanov

Kazan (Volga Region) Federal University

S. F Khaiboullina

Kazan (Volga Region) Federal University

S. A Lubin

City children’s Hospital №1

I. V Agapova

City children’s Hospital №1

References

  1. Camacho-Gonzales A., Spearman P.W., Stoll B.J. Neonatal infectious diseases: evaluation of neonatal sepsis. Pediatr. Clin. North. Am. 2013; 60: 367-89.
  2. Самсыгина Г.А. О предрасполагающих факторах и факторах риска развития неонатального сепсиса и о современных подходах его лечения Педиатрия 2012; 91 (3): 32-37
  3. Elmore S. Apoptosis: a review of programmed cell death. Toxicologic Pathology 2007; 35 (4): 495-516.
  4. Da Silva F.P., Nizet V. Cell death during sepsis: integration of disintegration in the inflammatory response to overwhelming infection. Apoptosis 2009; 14: 509-21.
  5. Hacker G. The morphology of apoptosis. Cell Tissue Res. 2000; 301: 5-17.
  6. Boomer J.S., To K., MD, Chang K.C. Immunosuppression in Patients Who Die of Sepsis and Multiple Organ Failure. JAMA 2011; 306(23): 2594-605
  7. Hotchkiss R.S., Tinsley K.W., Swanson P.E. et al. Sepsis-induced apoptosis causes progressive profound depletion of B and CD4+ T lymphocytes in humans. J. Immunol. 2001; 166: 6952-63.
  8. Hotchkiss R.S. , Tinsley K.W., Swanson P.E. et al. Depletion of dendritic cells, but not macrophages, in patients with sepsis. J. Immunol. 2002; 168: 2493-500.
  9. Hotchkiss R.S., Swanson P.E., Freeman B.D. et al. Apoptotic cell death in patients with sepsis, shock, andmultiple organ dysfunction. Crit. Care Med. 1999; 27: 1230-51.
  10. Hotchkiss R.S., Monneret G., Payen D. Immunosuppression in sepsis: novel understanding of the disorder and a new therapeutic approach. Lancet Infect Dis. 2013; 13: 260-8.
  11. Bochud P.Y., Calandra Th. Pathogenesis of sepsis: new concepts and implication for future treatment. BMJ 2003; 326(738): 262-5
  12. Kasten K.R., Tschöp J., Adediran S.G. et al. T cells are potent early mediators of the host response to sepsis. Shock 2010; 34(4): 327-36
  13. Хаертынов Х.С., Бойчук С.В. , Анохин В.А. и др. Апопотоз лимфоцитов у детей с неонатальным сепсисом. Каз. Мед. Жур. 2013; 94(5): 775-8.
  14. Хаертынов Х.С., Бойчук С.В., Анохин В.А. и др. Показатели активности апоптоза лимфоцитов крови у детей с неонатальным сепсисом. Гены и клетки 2014; 9 (3): 267-71.
  15. Riccardi C., Nicoletti I. Analysis of apoptosis by propidium iodide staining and flow cytometry. Nat. Protoc. 2006; 1(3): 1458-61
  16. Hotchkiss R.S., Coopersmith C.M., Karl I.E. Prevention of Lymphocyte Apoptosis-A Potential Treatment of Sepsis? Clin. Inf. Dis. 2005; 41: 465-9.
  17. Hotchkiss R.S., Karl I.E. The pathophysiology and treatment of sepsis. New Engl. J. med. 2003; 348(2): 138-50.
  18. Hargitai B., Szabó V., Hajdú J. et al. Apoptosis in Various Organs of Preterm Infants: Histopathologic Study of Lung, Kidney, Liver and Brain of Ventilated Infants. Pediatr. Res. 2001; 50(1): 110-4.

Copyright (c) 2015 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС 77 - 85657 от 21.07.2023 от 11.03.2014.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies