Lymphocyte apoptosis in neonatal sepsis due to klebsiella



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Abstract

The apoptosis of lymphocytes was examined in 10 infants with late neonatal sepsis induced by Klebsiella pneumoniae Most of the infants (70%) were preterm. Control group consisted of 7 healthy newborns. Apoptosis analysis was examined by counting the numbers of hypodiploid cells by using a propidium iodide (DNA staining procedure and flow cytometry analysis. We observed an elevated numbers of apoptotic cells in all cases of neonatal sepsis Median of lymphocyte apoptosis was 18 1% on day 5 of cell culture, whereas in control patients it was up to 7,8%. Absolute lymphopenia was observed in 30% of cases with neonatal sepsis. The activity of lymphocyte apoptosis was not associated with quantitative baseline of C-reactive protein (Spearman R = 0,48, p = 0,18). Acute phase of neonatal sepsis induced by Klebsiella pneumonia is associated with an increased apoptosis of peripheral blood lymphocytes

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About the authors

Kh. S Khaertynov

Kazan State Medical University

Email: khalit65@rambler.ru

S. V Boichuk

Kazan State Medical University

V. A Anokhin

Kazan State Medical University

B. R Ramazanov

Kazan State Medical University

A. A Rizvanov

Kazan (Volga Region) Federal University

S. F Khaiboullina

Kazan (Volga Region) Federal University

S. A Lubin

City children’s Hospital №1

I. V Agapova

City children’s Hospital №1

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