Role of K(ATP)-channels in the effects of hydrogen sulfide on the contractility of rat ventricular myocardium



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Abstract

The effect of sodium hydrosulfide (NaHS) - donor of hydrogen sulfide (H2S) on the force of contraction of isolated rat ventricle was studied. Application of NaHS in concentrations 1 and 10 μМ resulted in an increase and in concentrations 50, 100, 200 and 300 μМ - dose-dependent decrease of the force of contraction. Inhibition of K-channels by tetraethylammonium (15 mM) caused the increase of the amplitude of contraction and the negative inotropic effect of NaHS (200 μМ) was preserved. After the activation or inhibition of ATP-dependent K-channels by diazoxide (20 μМ) or glibenclamide (50 μМ), respectively, the negative inotropic effect of NaHS was the same as in control After preliminary application of NaHS glibenclamide (50 μМ) reversed the contraction force to yhe control values. The obtained data suppose that in the isolated rat ventricle exogenous H2S causes a reduction of the force of contraction, which is mediated by the activation of ATP-dependent K-channels

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About the authors

N. N Khaertdinov

Kazan (Volga region) Federal University

Email: Nail.Khaertdinov@kpfu.ru

A. S Lifanova

Kazan (Volga region) Federal University

A. R Gizzatullin

Kazan (Volga region) Federal University

G. F Sitdikova

Kazan (Volga region) Federal University

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