The morphological and phenotypic characteristics of microglia at different stages of cultivation and transplantation in the area of spinal cord injury in rats



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Abstract

The morphological and phenotypic characteristics of microglia were carried out immediately after isolation from the cerebral cortex of newborn rats, after 24 hours and 2 weeks of cultivation in vitro. It was shown that these cells expressed markers typical for microglia such as Iba1, CD68, CD11b/c, CD45 and Nestin throughout the cultivation period. Microglia had an amoeboid shape initially, but after 2 weeks branched forms of microglia appeared as well Freshly isolated microglia transduced with recombinant lentivirus LV-EGFP was transplanted to the site of spinal cord injury in rats. Transplanted cells survived in acute phase of injury for at least 14 days and expressed reporter EGFP

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About the authors

M. N Zhuravleva

Kazan (Volga region) Federal University

Y. O Mukhamedshina

Kazan (Volga region) Federal University

Email: yana.k-z-n@mail.ru

S. S Arkhipova

Kazan (Volga region) Federal University

E. R Sanatova

Kazan (Volga region) Federal University

A. A Rizvanov

Kazan (Volga region) Federal University

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