Deriving population of ectomesenchymal cells with properties of stem cells and progenitor cells from pulp of permanent teeth


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Abstract

Lately a lot of research has been focused on isolation of tooth stem cells. Our earlier studies showed that odontoblasts have a number of features typical for ectodermal cells, such as expression of epithelial membrane antigen (EMA) and cytokeratin 18. Aim: to obtain populations of ectomesenchymal cells from permanent teeth pulp with properties of stem and progenitor cells and characterize their phenotype in vitro. The pulp of permanent teeth of 15 patients (age 18- 29) was removed according to the orthodontic indications and used for stem cells isolation. The phenotype of derived cells was studied using the immunocytochemical staining method. Antibody staining for EMA, C-kit, cytokeratine 19, ESA, aSMA, Desmin, Vimentin, PCNA, Bcl-2. EMA and cytoceratine 18 expression was also confirmed by western blotting. The immunophenotype of obtained stem cells was determined by flow cytometry Significant expression of C-kit and Vimentin was observed at all times of cultivation as demonstrated by immunocytochemical staining. About a half of cells expressed EMA on all passages. First and second passages cells were characterized by expression of cytoceratine 18 which gradually decreased and to the fifth passage only single cells were positively stained. EMA and cytokeratin 18 expression was confirmed by western blotting. Flow cytometry revealed that cells obtained by us from human permanent teeth pulp were positive for CD29, CD90, CD10, CD54, CD56, CD166 and negative for CD14, CD34, CD45.

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About the authors

I. I Sagitov

Kazan State Medical University

A. K Shafigullina

Kazan (Volga region) Federal University

G. T Saleeva

Kazan State Medical University

M. O Gomzikova

Kazan (Volga region) Federal University

AA. A Rizvanov

Kazan (Volga region) Federal University

A. P Kiassov

Kazan (Volga region) Federal University

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