Deriving population of ectomesenchymal cells with properties of stem cells and progenitor cells from pulp of permanent teeth



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Abstract

Lately a lot of research has been focused on isolation of tooth stem cells. Our earlier studies showed that odontoblasts have a number of features typical for ectodermal cells, such as expression of epithelial membrane antigen (EMA) and cytokeratin 18. Aim: to obtain populations of ectomesenchymal cells from permanent teeth pulp with properties of stem and progenitor cells and characterize their phenotype in vitro. The pulp of permanent teeth of 15 patients (age 18- 29) was removed according to the orthodontic indications and used for stem cells isolation. The phenotype of derived cells was studied using the immunocytochemical staining method. Antibody staining for EMA, C-kit, cytokeratine 19, ESA, aSMA, Desmin, Vimentin, PCNA, Bcl-2. EMA and cytoceratine 18 expression was also confirmed by western blotting. The immunophenotype of obtained stem cells was determined by flow cytometry Significant expression of C-kit and Vimentin was observed at all times of cultivation as demonstrated by immunocytochemical staining. About a half of cells expressed EMA on all passages. First and second passages cells were characterized by expression of cytoceratine 18 which gradually decreased and to the fifth passage only single cells were positively stained. EMA and cytokeratin 18 expression was confirmed by western blotting. Flow cytometry revealed that cells obtained by us from human permanent teeth pulp were positive for CD29, CD90, CD10, CD54, CD56, CD166 and negative for CD14, CD34, CD45.

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About the authors

I. I Sagitov

Kazan State Medical University

A. K Shafigullina

Kazan (Volga region) Federal University

G. T Saleeva

Kazan State Medical University

M. O Gomzikova

Kazan (Volga region) Federal University

AA. A Rizvanov

Kazan (Volga region) Federal University

A. P Kiassov

Kazan (Volga region) Federal University

References

  1. Miura M., Gronthos S., Zhao M. et al. SHED: stem cells from human exfoliated deciduous teeth. PNAS USA 2003; 100: 5807-12.
  2. Ramazanoglu M., Rizvanov A.A., Sahin F. et al. Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neuro-genesis. Pharmacogenomics J. 2010; 10(2): 105-13.
  3. Morsczeck C., Gotz W., Schierholz J. et al. Isolation of precursor cells [PCs) from human dental follicle of wisdom teeth. Matrix. Biol. 2005; 24: 155-65.
  4. Gronthos S., Mankani M., Brahim J. et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. PNAS USA. 2000; 97: 13625-30.
  5. Prescott R.S., Alsanea R., Fayad M.I. et al. In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. Endod. 2008; 34(4): 421-6
  6. Komada Y., Yamane T., Kadota D. et al. Origins and properties of dental, thymic, and bone marrow mesenchymal cells and their stem cells. PLoSOne 2012; 7(11): e46436.
  7. Trubiani O., Di Primio R., Triani T. et al. Morphological and cytoflurometric analysis of adult mesenchymal stem cells expanded ex vivo from periodontal ligament. Int. J. Immunopathol. Pharmacol. 2005; 18: 213-21.
  8. Laino G., Graziano A., d'Aquino R. et al. An approachable human adult stem cell source for hard-tissue engineering. J. Cell Physiol. 2006; 206(3): 693-7.
  9. d'Aquino R., Graziano A., Sampaolesi M. et al. Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation. Cell Death Differ. 2007; 14(6): 1162-71.
  10. Шамсутдинов М.И. Стволовые и прогениторные клетки пульпы и их характеристики после ортопедического препарирования твердых тканей зуба. Автореферат дисс.. кандидата мед. наук. Казань: Казанский ГМУ, 2009.
  11. Соловьева В.В., Блатт Н.Л., Шафигуллина А.К., Ризванов. Исследование эндогенной секреции сосудистого эндотелиального фактора роста мультипотентными мезенхимными стромальными клетками из зачатков третьих моляров человека. Клеточная транс-плантол. и тканевая инженерия 2012; 7(3): 155-8.
  12. Alipour R., Sadeghi F., Hashemi-Beni B. Phenotypic characterizations and comparison of adult dental stem cells with adipose-derived stem cells. Int. J. Prev. Med. 2010; 1(3): 164-71.
  13. Orstavik D. Antibacterial properties of endodontic materials. Int. Endod J. 1988; 21: 61-169.
  14. Johnson W.B. A new guttapercha technique. J. Endod. 1978; 4: 184-8.
  15. Asmussen E., Peutzfeldt A., Heitmann Т. Stiffness, elastic limit, and strength of newer types of endodontic posts. J. Dent. 1999; 27: 275-8.
  16. Browne R.M. The in vitro assessment of the cytotoxicity of dental materials: does it have a role? Int. Endod. J. 1988; 21: 50-8.
  17. Cox C.F., Hafez A.A. Biocomposition and reaction of pulp tissues to restorative treatments. Dent. Clin. North Am. 2001; 45: 31-48.
  18. Ida K., Maseki T., Yamasaki M. et al. The pH values of pulp capping agents. J. Endod. 1989; 15: 365-8.
  19. Trubiani O., Di Primio R., Triani T. et al. Morphological and cytoflurometric analysis of adult mesenchymal stem cells expanded ex vivo from periodontal ligament. Int. J. Immunopathol. Pharmacol. 2005; 18: 213-21.
  20. Juskeviciute E., Vadigepalli R., Hoek J.B. Temporal and functional profile of the transcriptional regulatory network in the early regenerative response to partial hepatectomy in the rat. BMC Genomics 2008; 9: 527.
  21. Mafi P., Hindocha S., Mafi R. et al. Adult Mesenchymal Stem Cells and Cell Surface Characterization - A Systematic Review of the Literature. The Open Orthop. J. 2011; 5: 253-60.
  22. Dominici M., Le Blanc K., Dominici M. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006; 8(4): 315-7.
  23. Самцов А.В., Белоусова И.Э. Клинические, гистологические и иммуногистохимические особенности лимфом кожи на современном этапе. Вестник дерматологии и венерологии 2006; 1: 3-6.
  24. Kaukua N., Shahidi M.K., Konstantinidou C. et al. Glial origin of mesenchymal stem cells in a tooth model system. Nature 2014; 513(7519): 551-4.

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