Phenotypic modifications and quantitative analysis of glial cells in the area of spinal cord injury at the cell-mediated and direct gene delivery gdnf


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Abstract

On the model of rat spinal cord dosed contusion at T8 level studied the effect of delivery into the area of damage of the cell-mediated and direct gene delivery GDNF on maintaining a population of glial cells. Delivery into the area of damage adenoviral vector with gene GDNF (AdV-gdnf) using human umbilical cord blood mononuclear cells has a greater influence the amount of Ch47+-cells in the ventral horn (VH). Direct delivery of AdV-gdnf influences the amount of Ch47+-cells in dorsal roots entry zone (DREZ). Cell-mediated gene delivery GDNF causes the most pronounced changes in the expression of marker proteins astrocyte GFAP, S100B, and AQP4 in the ventral funiculus (VF) of white matter. Cell-mediated and direct delivery of gene GDNF support population GFAP+/S100B+-cells. The results indicate that the direct and cell-mediated gene delivery GDNF into spinal cord injury have different effects on the populations of glial cells in specific areas of spinal cord, that is important for the optimal method of delivery of therapeutic genes to stimulate posttraumatic neuroregeneration.

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Y. O Mukhamedshina

Kazan State Medical University; fazan (Volga region) Federal University

G. F Shaymardanova

Kazan Institute of Biochemistry and Biophysics of RAS

A. R Mukhitov

Kazan Institute of Biochemistry and Biophysics of RAS

E. E Garanina

fazan (Volga region) Federal University

A. A Rizvanov

fazan (Volga region) Federal University

Yu. A Chelyshev

Kazan State Medical University; fazan (Volga region) Federal University

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