Research of osteoplastic properties of matrixes from resolving polyether of hydroxioil acid


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Abstract

A family of extensions! implants with different composition was designed for reparative osteogenesis. The implants are made of a resorblng polymer of the hydroxybutyric acid (poly-3- hydroxybutyrate), a composition of this polymer with hydroxylapatite, and combination of poly-3-hydroxybutyrate with the recombinant human morphogenetic bone protein-2 (BMP-2). The properties of the implants developed were studied in experimental animals with segmental osteotomy in comparison with standard materials used in stomatology. Reconstructive osteogenesis has been shown to be active in all the implants containing poly-3-hydroxybutyrate as a main component. Poly-3- hydroxybutyrate itself as well as its compositions with hydroxylapatite and a morphogenetic protein BMP-2 have marked osteoplastic properties, degrade in vivo slowly and adequately to the growth of new bone tissue, promoting reparative osteogenesis.

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About the authors

E. I. Shishatskay

Institute of Biophysics, Siberian Branch of RAS; Department of Biotechnologies, Siberian Federal University

Author for correspondence.
Email: shishatskaya@inbox.ru
Russian Federation, Krasnoyarsk; Krasnoyarsk

I. V. Kamendov

Krasnoyarsk Stomatology Research Center for diabetes mellitus

Email: shishatskaya@inbox.ru
Russian Federation, Krasnoyarsk

S. I. Starosvetsky

Krasnoyarsk Stomatology Research Center for diabetes mellitus

Email: shishatskaya@inbox.ru
Russian Federation, Krasnoyarsk

T. G. Volova

Institute of Biophysics, Siberian Branch of RAS; Department of Biotechnologies, Siberian Federal University

Email: shishatskaya@inbox.ru
Russian Federation, Krasnoyarsk; Krasnoyarsk

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Supplementary files

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2. Fig. 1. Transverse cuts of bones in the area of the defect: I - PHB; II - PHB/HAP composite); III - PHA/gVMP-2; IV- "Bio-OSS®", V- "Collapan®". SW. ×25

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3. Fig. 2. Regenerate in the area of PHB implantation, 14 days: A - proliferation of osteogenic tissue around PHB fragments (1); B - newly formed Haversian systems. Staining: hematoxylin and eosin. SW: A - ×100; B - ×400

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4. Fig. 3. Regenerate in the area of PHB/HAP implantation, 14 days: A - newly formed bone beams built from immature bone tissue; B — a fragment of the implanted material surrounded by reactively altered connective tissue and bone beams. Staining: hematoxylin and eosin. SW. ×100

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5. Fig. 4. Regenerate in the area of implantation of PHA/rhBMP-2, 14 days: A - field of cartilage tissue, which is a springboard for the deployment of enchondral osteogenesis; B - implant fragments surrounded by reactively altered connective tissue. Staining: hematoxylin and eosin. SW. ×100

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6. Fig. 5. Regenerate in the area of implantation of the Collapol® preparation, 14 days: A — disordered bone trabeculae and fragments of resorbable material; B - a fragment of the Collapol® material surrounded by osteogenic tissue. Staining: hematoxylin and eosin. SW. ×100

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7. Fig. 6. Regenerate in the area of implantation of the preparation "Bio-OSS®", 14 sec.: reactive growths of mineralized tissue around the resorbable components of the material. Staining: hematoxylin and eosin. SW. ×100

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