Generation of recombinant adenoviruses and lentiviruses expressing angiogenic and neuroprotectivefactors using Gateway cloning technology
- Authors: Cherenkova EE1, Fedotova VY1, Borisov MA2, Islamov RR2, Rizvanov AA1
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Affiliations:
- Kazan (Volga region) Federal University, Kazan
- Kazan State Medical University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 164-168
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121687
- DOI: https://doi.org/10.23868/gc121687
- ID: 121687
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Abstract
find an optimal vector - a carrier of genetic information.
Viruses represent a natural biological system for gene
transfer into eukaryotic cells. One of the most effective
and proven vectors for delivery of recombinant nucleic acids
into mammalian cells are adenoviruses and lentiviruses. In
this study using the Gateway cloning we have constructed
adenoviral and lentiviral vectors encoding angiogenic and
neuroprotective factors: various isoforms of vascular
endothelial growth factor vegf121, vegf165, vegf189; basic
fibroblast growth factor fgf2; glial cell-derived neurotrophic
factor gdnf. The efficiency of transduction of HEK293A cell
line with generated recombinant viruses and expression of
recombinant proteins were confirmed by immunofluorescent
analysis.
About the authors
E E Cherenkova
Kazan (Volga region) Federal University, KazanKazan (Volga region) Federal University, Kazan
V Y Fedotova
Kazan (Volga region) Federal University, KazanKazan (Volga region) Federal University, Kazan
M A Borisov
Kazan State Medical University, KazanKazan State Medical University, Kazan
R R Islamov
Kazan State Medical University, KazanKazan State Medical University, Kazan
A A Rizvanov
Kazan (Volga region) Federal University, KazanKazan (Volga region) Federal University, Kazan
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Supplementary files
