Kompozitsionnye matritsy na osnove khitina i khitozana dlya kul'tivirovaniya kletok kozhi cheloveka



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详细

A set of composite films based on chitosan or chitosan-collagen mixtures are developed to be used as the resorbable matrices for fibroblastes and keratinocytes cultivation, and optimal conditions of their formation are precised. 3D porous matrices are obtained to insure the simultaneous cultivation of both types of cells. The composite materials prepared by this method in forms of films and sponges were characterized and then tested as the matrices for cell cultivation to obtain the transplantats for wound healing. Both in vitro and in vivo tests of all materials under study have evidenced the absence of any toxic actions on the cultivated cells and on the tissues of organism. The matrices are stable in the conditions of cultivation and resorb completely in in vivo conditions in 10 days after the transplantation.

作者简介

E Panarin

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg 2 the Institute of Cytology, RAS, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg 2 the Institute of Cytology, RAS, Saint-Petersburg

L Nud'ga

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

V Petrova

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

A Bochek

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

I Goffman

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

Yu Baklagina

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

N Saprykina

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

the Institute of Macromolecular Compounds, RAS, Saint-Petersburg

M Blinova

the Institute of Cytology, RAS, Saint-Petersburg

the Institute of Cytology, RAS, Saint-Petersburg

N Yudintseva

the Institute of Cytology, RAS, Saint-Petersburg

the Institute of Cytology, RAS, Saint-Petersburg

O Spichkina

the Institute of Cytology, RAS, Saint-Petersburg

the Institute of Cytology, RAS, Saint-Petersburg

L Kukhareva

the Institute of Cytology, RAS, Saint-Petersburg

the Institute of Cytology, RAS, Saint-Petersburg

I Samusenko

A.M. Nikiforov National Center of Urgent and Radiation Medicine, EMERCOM of Russia, Saint-Petersburg

A.M. Nikiforov National Center of Urgent and Radiation Medicine, EMERCOM of Russia, Saint-Petersburg

G Pinaev

the Institute of Cytology, RAS, Saint-Petersburg

the Institute of Cytology, RAS, Saint-Petersburg

参考

  1. Панарин Е.Ф., Нудьга Л.А., Петрова В.А. и соавт. Матрицы для культивирования клеток кожи человека на основе природных полисахаридов - хитина и хитозана. Клеточная трансплантология и тканевая инженерия 2009; IVC3]: 42-6.
  2. Terbojevich М., Corrado С, Cosani A., Marsano Е. Solution studies the chitin - lithium chloride - N,N-dimethylacetamide system. Carbohydr. Res. 1988; 180:73-86.
  3. Chandrakasan G., Torchia D.A., Piez K.H.. Preparation of intact monomeric collagen from rat tail tendon and skin and the structure of the helical ends in solution. J. Biol. Chem. 1976; 251(19]: 6062-7.
  4. Хилькин A.M., Шехтер А.Б., Истранов Л.П., Леменев В.Л. Коллаген и его применение в медицине. М.: Медицина 1976: 228.
  5. Михайлов Г.М., Лебедева М.Ф., Нудьга Л.А. и др. Структура и механические свойства волокон из хитина, модифицированных эфирами целлюлозы. Материалы Пятой конф. «Новые перспективы в исследовании хитина и хитозана». Москва-Щелково 1999: 51.

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